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The role of liposome size on the type of immune response induced in BALB/c mice against leishmaniasis: rgp63 as a model antigen.

Abstract : To develop an efficient liposomal vaccine delivery system, the size of liposomes is critical to their adjuvant activities. In the present study, liposomes with different sizes (100, 400, 1000nm) containing recombinant major surface glycoprotein of Leishmania (rgp63) were prepared, characterized, and inoculated subcutaneously into BALB/c mice to evaluate the rate of protection and the type of immune response generated against leishmaniasis. The lowest footpad lesion size and splenic parasite burden were seen in the mice immunized with large size (⩾400nm) liposomes after challenge with Leishmania major. The production of IFN-γ was only elevated in the spleen cells of mice immunized with large size (⩾400nm) liposomes. The highest IgG2a/IgG1 ratio was also seen in the sera of the mice immunized with large size (⩾400nm) liposomes before and 14weeks after challenge. The results showed that immunization with small size (100nm) liposomes induces a Th2 response, whereas immunization with large size (⩾400nm) liposomes induces a Th1 type of immune response. There was no significant difference in the type of induced immune response between the mice immunized with liposomes of 400nm and those immunized with liposomes of 1000nm or unextruded. The results of the current study demonstrated that the size of liposomes plays a significant role in the type of generated immune response.
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Submitted on : Tuesday, October 30, 2012 - 2:03:54 PM
Last modification on : Wednesday, October 14, 2020 - 4:08:06 AM
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Ali Badiee, Ali Khamesipour, Afshin Samiei, Dina Soroush, Vahid Heravi Shargh, et al.. The role of liposome size on the type of immune response induced in BALB/c mice against leishmaniasis: rgp63 as a model antigen.. Experimental Parasitology, Elsevier, 2012, 4894 (12), pp.261-5. ⟨10.1016/j.exppara.2012.09.001⟩. ⟨pasteur-00747050⟩

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