Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells.

Abstract : BACKGROUND: The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic properties of HESA-A are able to treat psoriasis vulgaris and cancers. However, no antiviral properties have been reported. METHODS: This study was designed to investigate the potential antiviral properties of HESA-A and its effects in modulating TNF-α and IL-6 cytokine levels. HESA-A was prepared in normal saline as a stock solution (0.8 mg/ml, pH = 7.4). Percentages of cell survival when exposed to different concentrations of HESA-A at different time intervals was determined by MTT assay. To study the potential antiviral activity of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were treated with the effective concentration (EC50) of HESA-A (0.025 mg/ml) and 100 TCID50/0.1 ml of virus sample under different types of exposure. RESULTS: Based on the MTT method and hemagglutination assay (HA), HESA-A is capable of improving cell viability to 31% and decreasing HA titre to almost 99% in co-penetration exposures. In addition, based on quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), it was found that HESA-A causes decrements in TNF-α and IL-6 cytokine expressions, which was significant for TNF-α (p ≤ 0.05) but not for IL-6. CONCLUSION: In conclusion, HESA-A was effective against influenza infection through suppressing cytokine expression.
Document type :
Journal articles
Complete list of metadata

Cited literature [25 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00747078
Contributor : Masoumeh Tavassoti Kheiri Connect in order to contact the contributor
Submitted on : Tuesday, October 30, 2012 - 2:34:14 PM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM
Long-term archiving on: : Thursday, January 31, 2013 - 3:47:05 AM

File

HESA_article.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Parvaneh Mehrbod, Aini Ideris, Abdul Rahman Omar, Mohd Hair-Bejo, Sheau Wei Tan, et al.. Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells.. Virology Journal, BioMed Central, 2012, 9, pp.44. ⟨10.1186/1743-422X-9-44⟩. ⟨pasteur-00747078⟩

Share

Metrics

Record views

51

Files downloads

338