Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Enhanced production of 2,3-butanediol from glycerol by forced pH fluctuations.

Abstract : The glycerol fermentation by Klebsiella pneumoniae occurs by receiving more than five liquid products-organic acids, diols, and ethanol. Aiming to direct the glycerol conversion towards predominant production of 2,3-butanediol (2,3-BD), the main influencing parameters (the aeration and the pH) were investigated during fed-batch processes. The regime of intensive aeration (2.2 vvm air supply) was evaluated as most favorable for 2,3-BD synthesis and ensured the decrease of all other metabolites. Thus, without pH control, 52.5 g/l 2,3-BD were produced, as the carbon conversion of glycerol into 2,3-BD reached 60.6%. Additional enhancement in 2,3-BD production (by significant increase of glycerol utilization) was achieved by the development of a new method of "forced pH fluctuations". It was realized by consecutive raisings of pH using definite DeltapH value, at exact time intervals, allowing multiple variations. Thus, the optimal conditions for maximal glycerol consumption were defined, and 70 g/l 2,3-BD were produced, which is the highest amount obtained from glycerol as a sole carbon source until now. The forced pH fluctuations emphasized pH as a governing factor in microbial conversion processes.
Document type :
Journal articles
Complete list of metadata

Cited literature [18 references]  Display  Hide  Download
Contributor : Petar Grozdanov Connect in order to contact the contributor
Submitted on : Monday, November 5, 2012 - 12:11:23 PM
Last modification on : Friday, September 6, 2019 - 11:14:03 AM
Long-term archiving on: : Saturday, December 17, 2016 - 7:44:45 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Kaloyan Petrov, Penka Petrova. Enhanced production of 2,3-butanediol from glycerol by forced pH fluctuations.. Applied Microbiology and Biotechnology, Springer Verlag, 2010, 87 (3), pp.943-9. ⟨10.1007/s00253-010-2545-z⟩. ⟨pasteur-00748327⟩



Record views