Abstract : Isolate A-3 from Antarctic soil in Casey Station, Wilkes Land, was characterized for growth on hydrocarbons. Use of glucose or kerosene as a sole carbon source in the culture medium favoured biosynthesis of surfactant which, by thin-layer chromatography, indicated the formation of a rhamnose-containing glycolipid. This compound lowered the surface tension at the air/water interface to 27 mN/m as well as inhibited the growth of B. subtilis ATCC 6633 and exhibited hemolytic activity. A highly hydrophobic surface of the cells suggests that uptake occurs via a direct cell-hydrocarbon substrate contact. Strain A-3 is Gram-positive, halotolerant, catalase positive, urease negative and has rod-coccus shape. Its cell walls contained meso-diaminopimelic acid. Phylogenetic analysis based on comparative analysis of 16S rRNA gene sequences revealed that strain A-3 is closely related to Rhodococcus fascians with which it shares 100% sequence similarity. This is the first report on rhamnose-containing biosurfactant production by Rhodococcus fascians isolated from Antarctic soil.
https://hal-riip.archives-ouvertes.fr/pasteur-00748956
Contributor : Petar Grozdanov <>
Submitted on : Tuesday, November 6, 2012 - 1:03:43 PM Last modification on : Wednesday, March 13, 2019 - 1:52:39 PM Long-term archiving on: : Saturday, December 17, 2016 - 7:40:27 AM
File
Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed
until : jamais