Direct identification of non-polio enteroviruses in residual paralysis cases by analysis of VP1 sequences

Pooneh Rahimi (1) , Hamideh Tabatabie (2) , Mohammad Mehdi Gouya (3) , Mahmoud Mahmoudi (2) , Taha Musavi (3) , Katayoun Samimi Rad (2) , Talaat Mokhtariazad (2) , Rakhashandeh Nategh (2)

1 Department of Virology
2 PNL - Polio National Laboratory,Teheran University of Medical Sciences
3 The Disease Management Center of Ministry of Health

Background: The 66 serotypes of human enteroviruses (EVs) are classified into four species A-D, based on phylogenetic relationships in multiple genome regions. Partial VP1 amplification and sequence analysis are reliable methods for identifying non-polio enterovirus serotypes, especially in negative cell culture specimens from patients with residual paralysis. Objectives: In Iran during the years 2000-2002, there were 29 residual paralysis cases with negative cell (RD, HEp2 and L20B) culture results. Study design: The genomic RNA was extracted from stool specimens from cases of residual paralysis and detected by amplification of the 5 -nontranslated region using RT-PCR with Pan-EV primers. Partial VP1 amplification by semi-nested RT-PCR (snRT-PCR) and sequence analysis were done. Results: Specimens from the 29 culture-negative cases contained echoviruses of six different serotypes. Conclusions: The global eradication of wild polioviruses is near and study of non-polio enteroviruses, which can cause poliomyelitis, is increasingly important to understand their pathogenesis. The VP1 sequences, derived from the snRT-PCR products, allowed rapid molecular analysis of these non-polio strains.

Mots clés: Non-polio enteroviruses; Echoviruses; Residual paralysis

Domaines: Virologie

Pooneh Rahimi, Hamideh Tabatabie, Mohammad Mehdi Gouya, Mahmoud Mahmoudi, Taha Musavi, et al.. Direct identification of non-polio enteroviruses in residual paralysis cases by analysis of VP1 sequences. Journal of Clinical Virology, 2009, 45 (2), pp.139-141. ⟨10.1016/j.jcv.2009.03.004⟩. ⟨pasteur-00750588⟩