Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Immune responses elicited by co-immunization of Plasmodium vivax and P. falciparum MSP-1 using prime-boost immunization strategies.

A. A. Mehrizi 1 S. Zakeri 1, * S. Rafati 1 A. H. Salmanian 1 N. D. Djadid 1 
* Corresponding author
Abstract : Carboxy-terminus of merozoite surface protein-1 (MSP-1(19) ) is the major protein on the surface of the plasmodial merozoite that acts as one of the most important blood-stage vaccine candidates. The present investigation was designed to evaluate the immune responses when either two recombinant antigens (rPvMSP-1(19) + rPfMSP-1(19)) or two plasmid constructs (pcDNA3.1 hygro-PvMSP-1(19) + pcDNA3.1 hygro-PfMSP-1(19)) were administered in combination at a single site in mice by using different immunization strategies (protein/protein, DNA/DNA and DNA/protein) at weeks 0, 5 and 8. All mice were monitored for the level of MSP-1(19) -specific antibody for up to 40 weeks. The inclusion of both recombinant antigens in a vaccine mixture could not inhibit induction of antibodies to the other antigen when the two recombinant antigens were combined in immunization formulation. Interestingly, antisera from immunized mice with either recombinant antigen failed to cross-react with heterologous antigen. Moreover, the results of this study showed that co-immunization with both antigens at a single site generated a substantial PvMSP-1(19) - and PfMSP-1(19) -specific antibody responses and also IFN-γ cytokine production (Th1 response) in DNA/protein prime-boost immunization strategies. The increased humoral response to PvMSP-1(19) and PfMSP-1(19) lasted nearly a year after immunization. Therefore, the results of this study are encouraging for the development of multi-species malaria vaccine based on MSP-1(19) antigen.
Document type :
Journal articles
Complete list of metadata

Cited literature [29 references]  Display  Hide  Download
Contributor : Akram Abouei Mehrizi Connect in order to contact the contributor
Submitted on : Wednesday, November 14, 2012 - 12:15:36 PM
Last modification on : Wednesday, October 14, 2020 - 3:43:51 AM
Long-term archiving on: : Saturday, December 17, 2016 - 10:24:55 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




A. A. Mehrizi, S. Zakeri, S. Rafati, A. H. Salmanian, N. D. Djadid. Immune responses elicited by co-immunization of Plasmodium vivax and P. falciparum MSP-1 using prime-boost immunization strategies.. Parasite Immunology, Wiley, 2011, 33 (11), pp.594-608. ⟨10.1111/j.1365-3024.2011.01331.x⟩. ⟨pasteur-00751829⟩



Record views