Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus.

Vahid Khalaj (1) , Hamid Eslami (1) , Mohammad Azizi (1) , Nuria Rovira-Graells (2) , Mike Bromley (2)

1 Biotechnology Research Center
2 F2g

An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs.

Keywords: AMA1; RNAi; Aspergillus; cbhB

Domains: Life Sciences [q-bio]; Biotechnology; Computer Science [cs]; Biotechnology

Vahid Khalaj, Hamid Eslami, Mohammad Azizi, Nuria Rovira-Graells, Mike Bromley. Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus.. FEMS Microbiology Letters, 2007, 270 (2), pp.250-4. ⟨10.1111/j.1574-6968.2007.00680.x⟩. ⟨pasteur-00753086⟩