Screening of the anticonvulsant activity of some plants from Fabaceae family in experimental seizure models in mice.

Abstract : BACKGROUND AND PURPOSE OF THE STUDY: Fabaceae is the third largest family of flowering plants. Lack of essential oils in the plants of this family can be an advantage in search for safe and effective medicines. In this study the anticonvulsant effect of the leaves of Albizzia julibrissin, Acacia juliflora, Acacia nubica and aerial parts of Astragalus obtusifolius was evaluated in pentylenetetrazole (PTZ) and maximal electroshock (MES) seizure tests. METHODS: The hydroalcoholic extracts of the plants were obtained by percolation. Different doses of the extracts were injected to the mice intraperitoneally (i.p.) and occurrence of clonic seizures induced by PTZ (60 mg/kg, i.p.) or tonic seizures induced by MES (50 mA, 50Hz, 1sec) were monitored up to 30 min after administration. Acute toxicity of the extracts was also assessed. The safe and effective extract was then fractionated by dichloromethane and anticonvulsant activity of the fractions was determined. Finally, the constituents of the extract and the fractions were screened by thin layer chromatography. RESULTS: Among the extracts, only A. obtusifolius extract showed low toxicity and protective effect against clonic seizures with ED50 value of 3.97 g/kg. Fractionation of the extract led to increase in anticonvulsant activity and ED50 value of 2.86 g/kg was obtained for the aqueous fraction. Phytochemical screening revealed the presence of alkaloids, flavonoids, anthrones and saponins in the aqueous fraction. MAJOR CONCLUSION: The presence of anticonvulsant compounds in A. obtusifolius suggests further activity-guided fractionation and analytical studies to find out the potential of this plant as a source of anticonvulsant agent.
Document type :
Journal articles
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00753192
Contributor : Soroush Sardari <>
Submitted on : Tuesday, January 15, 2013 - 7:00:15 AM
Last modification on : Thursday, September 19, 2019 - 1:41:44 PM

File

11.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

M. Sayyah, A. Khodaparast, A. Yazdi, S. Sardari. Screening of the anticonvulsant activity of some plants from Fabaceae family in experimental seizure models in mice.. DARU Journal of Pharmaceutical Sciences, Tehran University of Medical Sciences, 2011, 19 (4), pp.301-5. ⟨pasteur-00753192⟩

Share

Metrics

Record views

130

Files downloads

55