Potential molecular targets in chemopreventative action of celecoxib: a proteomics analysis of J774.A1 macrophage-like cell line.

Faegheh Rezaie (1) , Mona Salimi (2) , Mohammad Hossein Ghahremani (1) , Behrouz Vaziri (3)

1 Toxicology and Pharmacology Department, Faculty of Pharmacy
2 Institut Pasteur d'Iran
3 Protein Chemistry Unit, Biotechnology Research Center

The overexpression of cyclooxygenase-2 (COX-2) enzyme has been strongly contributed to tumorigenesis. The efficacy of celecoxib as a selective COX-2 inhibitor has been shown in many studies, but the underlying mechanism as a chemopreventative agent has not yet been well known. For better understanding the chemopreventative molecular mechanisms, we used a comparative proteomics analysis of lipopolysaccharide (LPS) treated and untreated J774.A1 macrophage-like cell lines before and after treatment with celecoxib. Our findings define the contribution of several interesting proteins, including ferritin heavy chain, glyoxalase-1, cofilin, vimentin, and galectin-1, which could extend our understanding of the chemopreventative effects of celecoxib and provide new valuable tools for further anticancer research.

Keywords: chemopreventative action; celecoxib; proteomics; J774.A1; cell line

Domains: Immunology

Faegheh Rezaie, Mona Salimi, Mohammad Hossein Ghahremani, Behrouz Vaziri. Potential molecular targets in chemopreventative action of celecoxib: a proteomics analysis of J774.A1 macrophage-like cell line.. Molecular BioSystems, 2011, 7 (4), pp.1306-11. ⟨10.1039/c0mb00201a⟩. ⟨pasteur-00757075⟩