[Qnr-type quinolone resistance in extended-spectrum beta-lactamase producing enterobacteria in Abidjan, Ivory Coast].

N. Guessennd (1) , S. Bremont (2) , V. Gbonon (1) , A. Kacou-Ndouba (1) , E. Ekaza (1) , T. Lambert (2) , M. Dosso (1) , P. Courvalin (2)

1 Laboratoire de bactériologie-virologie
2 Agents antibactériens

The aim of the study was to show the emergence of the qnr genes in extended spectrum beta-lactamases producing enterobacteria in Abidjan between 2005 and 2006. The whole of 151 strains of extended spectrum beta-lactamases producing enterobacteria were studied: 64 Escherichia coli, 66 Klebsiella pneumoniae, seven Klebsiella oxytoca and 14 Enterobacter spp. isolated from various biological products and from in- and out-patients. The techniques of disks diffusion, double-disk synergy, E-test were respectively used for the antimicrobial susceptibility test, the detection of extended spectrum beta-lactamases and the minimal inhibiting concentration. The bla genes(SHV, TEM, CTXM groups 1, 2, 8, 9), and AmpC were determined by PCR and characterized by sequencing. A global prevalence of 27,2 % (41/151) and rates of 9,9, 14,6, 2,7 % for the qnr genes A, B, A and S were observed. The distribution was 42,9 % for Enterobacter spp, 31,2 % for Escherichia coli, 20,5 % for Klebsiella; 30 strains expressed at least two bla genes; four strains were associated with AmpC. The strains were resistant to the cotrimoxazole (97,6 %), to the céfépime (73,2 %), to the céfoxitine (56,1 %), to the imipénème (0 %) and 43,9 % to all the aminosides. This high qnr gene prevalence associated with several types of bla genes in epidemic matter, the high level of resistance to antibiotics make fear a high risk of the transmission of multi-resistants bacteria and challenge the authorities for a resistance monitoring policy.

Mots clés: qnr; Quinolones; Résistance; Entérobactéries; Bêta-lactamases à spectre élargi

Domaines: Bactériologie

N. Guessennd, S. Bremont, V. Gbonon, A. Kacou-Ndouba, E. Ekaza, et al.. [Qnr-type quinolone resistance in extended-spectrum beta-lactamase producing enterobacteria in Abidjan, Ivory Coast].. Pathologie Biologie, 2008, 56 (7-8), pp.439-46. ⟨10.1016/j.patbio.2008.07.025⟩. ⟨pasteur-00758944⟩