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Journal articles
Helicobacter pylori Omp18 and its application in serologic screening of infection.
Abstract : Helicobacter pylori (Hp) is a major risk factor for gastrointestinal disorders including gastric cancer. We evaluated host serum antibody responses toward outer membrane protein18 in comparison with Urease A and B subunits. omp18 and ureA-ureB gene fragments were PCR amplified, cloned, and expressed in E. coli expression system. The expressed proteins were visualized on SDS-PAGE and confirmed by immuno-blotting. Purified proteins were applied in western blotting assays in comparison with local and foreign ELISA kits. ROC curve analysis identified the optimum cut-off points for each protein. rOmp18 represented the highest rates of sensitivity (94%), specificity (89%), PPV (97.4%), NPV (77.4%), and accuracy (93.2%) in comparison with urease A and B subunits. These immunologic indices were in "substantial" agreement (Κ = 0.7) with the gold standard tests for Hp detection. This study recommends Hp conserved Omp18 as a reliable serologic marker for accurate detection of Hp infection particularly for application in population screening approaches.
Cited literature [22 references]
https://hal-riip.archives-ouvertes.fr/pasteur-00762907
Contributor : Marjan Mohammadi <>
Submitted on : Sunday, December 9, 2012 - 7:05:02 AM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM
Long-term archiving on: : Saturday, December 17, 2016 - 10:57:10 PM
Contributor : Marjan Mohammadi <>
Submitted on : Sunday, December 9, 2012 - 7:05:02 AM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM
Long-term archiving on: : Saturday, December 17, 2016 - 10:57:10 PM