Helicobacter pylori Omp18 and its application in serologic screening of infection. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles Current Microbiology Year : 2011

Helicobacter pylori Omp18 and its application in serologic screening of infection.

Abstract

Helicobacter pylori (Hp) is a major risk factor for gastrointestinal disorders including gastric cancer. We evaluated host serum antibody responses toward outer membrane protein18 in comparison with Urease A and B subunits. omp18 and ureA-ureB gene fragments were PCR amplified, cloned, and expressed in E. coli expression system. The expressed proteins were visualized on SDS-PAGE and confirmed by immuno-blotting. Purified proteins were applied in western blotting assays in comparison with local and foreign ELISA kits. ROC curve analysis identified the optimum cut-off points for each protein. rOmp18 represented the highest rates of sensitivity (94%), specificity (89%), PPV (97.4%), NPV (77.4%), and accuracy (93.2%) in comparison with urease A and B subunits. These immunologic indices were in "substantial" agreement (Κ = 0.7) with the gold standard tests for Hp detection. This study recommends Hp conserved Omp18 as a reliable serologic marker for accurate detection of Hp infection particularly for application in population screening approaches.
Embargoed file
Embargoed file
Ne sera jamais visible
Loading...

Dates and versions

pasteur-00762907 , version 1 (09-12-2012)

Identifiers

Cite

Yeganeh Talebkhan, Fatemeh Ebrahimzadeh, Maryam Esmaeili, Leili Zamaninia, Azin Nahvijoo, et al.. Helicobacter pylori Omp18 and its application in serologic screening of infection.. Current Microbiology, 2011, 62 (1), pp.325-30. ⟨10.1007/s00284-010-9694-2⟩. ⟨pasteur-00762907⟩

Collections

RIIP RIIP_IRAN
48 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More