Helicobacter pylori Omp18 and its application in serologic screening of infection.

Yeganeh Talebkhan (1) , Fatemeh Ebrahimzadeh (1) , Maryam Esmaeili (1) , Leili Zamaninia (1) , Azin Nahvijoo (2) , Hossein Khedmat (3) , Forouzandeh Fereidooni (2) , Mohammad Ali Mohagheghi (2) , Marjan Mohammadi (1)

1 Biotechnology Research Center
2 Cancer Research Center
3 Baqiyatallah Research Center for Gastroenterology and Liver Diseases

Helicobacter pylori (Hp) is a major risk factor for gastrointestinal disorders including gastric cancer. We evaluated host serum antibody responses toward outer membrane protein18 in comparison with Urease A and B subunits. omp18 and ureA-ureB gene fragments were PCR amplified, cloned, and expressed in E. coli expression system. The expressed proteins were visualized on SDS-PAGE and confirmed by immuno-blotting. Purified proteins were applied in western blotting assays in comparison with local and foreign ELISA kits. ROC curve analysis identified the optimum cut-off points for each protein. rOmp18 represented the highest rates of sensitivity (94%), specificity (89%), PPV (97.4%), NPV (77.4%), and accuracy (93.2%) in comparison with urease A and B subunits. These immunologic indices were in "substantial" agreement (Κ = 0.7) with the gold standard tests for Hp detection. This study recommends Hp conserved Omp18 as a reliable serologic marker for accurate detection of Hp infection particularly for application in population screening approaches.

Keywords: Helicobacter pylori; Omp18; Serologic; Screening; Infection

Domains: Computer Science [cs]; Biotechnology; Life Sciences [q-bio]; Biotechnology

Yeganeh Talebkhan, Fatemeh Ebrahimzadeh, Maryam Esmaeili, Leili Zamaninia, Azin Nahvijoo, et al.. Helicobacter pylori Omp18 and its application in serologic screening of infection.. Current Microbiology, 2011, 62 (1), pp.325-30. ⟨10.1007/s00284-010-9694-2⟩. ⟨pasteur-00762907⟩