S. R. Bergmann, K. A. Fox, M. M. Ter-pogossian, B. E. Sobel, and D. Collen, Clot-selective coronary thrombolysis with tissue-type plasminogen activator, Science, vol.220, issue.4602, pp.220-1181, 1983.
DOI : 10.1126/science.6602378

F. Van-de-werf, S. R. Bergmann, K. A. Fox, H. De-geest, C. F. Hoyng et al., Coronary thrombolysis with intravenously administered human tissue-type plasminogen activator produced by recombinant DNA technology, Circulation, vol.69, issue.3, pp.69-605, 1984.
DOI : 10.1161/01.CIR.69.3.605

F. Van-de-werf, P. A. Ludbrook, S. R. Bergmann, A. J. Tiefenbrunn, K. A. Fox et al., Coronary thrombolysis with tissue-type plasminogen activator in patients with evolving myocardial infarction, pp.310-609, 1984.

S. Z. Goldhaber, Pulmonary thromboembolism, Harrison's Principles of Internal Medicine, pp.1561-1565, 2005.

G. P. Clagett, M. Sobel, M. R. Jackson, G. Y. Lip, M. Tangelder et al., Antithrombotic therapy in peripheral arterial occlusive disease: the seventh ACCP conference on antithrombotic and thrombolytic therapy, Chest, issue.3, pp.126-609, 2004.

C. J. Murray and A. D. Lopez, Mortality by cause for eight regions of the world: Global Burden of Disease Study, The Lancet, vol.349, issue.9061, pp.349-1269, 1997.
DOI : 10.1016/S0140-6736(96)07493-4

S. Z. Goldhaber, D. E. Vaughan, J. E. Markis, A. P. Selwyn, M. F. Meyerovitz et al., ACUTE PULMONARY EMBOLISM TREATED WITH TISSUE PLASMINOGEN ACTIVATOR, The Lancet, vol.328, issue.8512, pp.886-889, 1986.
DOI : 10.1016/S0140-6736(86)90411-3

R. A. Graor, Tissue Plasminogen Activator, Thrombolytic therapy, pp.171-207, 1987.

H. K. Gold, J. A. Johns, R. C. Leinbach, T. Yasuda, E. Grossbard et al., A randomized, blinded, placebo-controlled trial of recombinant human tissue-type plasminogen activator in patients with unstable angina pectoris, Circulation, vol.75, issue.6, pp.75-1192, 1987.
DOI : 10.1161/01.CIR.75.6.1192

D. Belin, J. D. Vassalli, C. Combepine, F. Godeau, Y. Nagamine et al., Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator, European Journal of Biochemistry, vol.224, issue.2, pp.225-232, 1985.
DOI : 10.1016/0022-2836(84)90192-X

A. M. Ross, New plasminogen activators: A clinical review, Clinical Cardiology, vol.19, issue.3, pp.165-171, 1999.
DOI : 10.1002/clc.4960220303

M. L. Simoons, P. W. Serruys, M. Van-den-brand, F. Bar, C. De-zwaan et al., Improved survival after early thrombolysis in acute myocardial infarction. A randomised trial by the interuniversity cardiology institute in The Netherlands, Lancet, issue.28455, pp.578-82, 1985.

D. Pennica, W. E. Holmes, W. J. Kohr, R. N. Harkins, G. A. Vehar et al., Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli, Nature, vol.2, issue.5897, pp.301-214, 1983.
DOI : 10.1038/301214a0

S. J. Szarka, E. G. Sihota, H. R. Habibi, and S. Wong, Staphylokinase as a plasminogen activator component in recombinant fusion proteins, Applied and Environmental Microbiology, vol.65, issue.2, pp.506-513, 1999.

J. B. Griffiths and A. Electricwala, Production of tissue plasminogen activators from animal cells, Advances in Biochemical Engineering/Biotechnology, vol.34, pp.147-166, 1987.
DOI : 10.1007/BFb0000678

J. Qiu, J. R. Swartz, and G. Georgiou, Expression of active human tissue-type plasminogen activator in escherichia coli, Applied and Environmental Microbiology, vol.64, issue.12, pp.4891-4896, 1998.

E. Martegani, N. Forlani, I. Mauri, D. Porro, W. D. Schleuning et al., Expression of high levels of human tissue plasminogen activator in yeast under the control of an inducible GAL promoter, Applied Microbiology and Biotechnology, vol.37, issue.5, pp.604-608, 1992.
DOI : 10.1007/BF00240734

P. H. Bessette, F. Aslund, J. Beckwith, and G. Georgiou, Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm, Proceedings of the National Academy of Sciences, vol.96, issue.24, pp.96-13703, 1999.
DOI : 10.1073/pnas.96.24.13703

C. E. White, N. M. Kempi, and E. A. Komives, Expression of highly disulfide???bonded proteins in Pichia pastoris, Structure, vol.2, issue.11, pp.1003-1005, 1994.
DOI : 10.1016/S0969-2126(94)00103-0

J. M. Cregg, T. S. Vedvick, and W. C. Raschke, Recent Advances in the Expression of Foreign Genes in Pichia pastoris, Bio/Technology, vol.186, issue.8, pp.11-905, 1993.
DOI : 10.1146/annurev.bi.56.070187.004411

K. N. Faber, W. Harder, G. Ab, and M. Veenhuis, Review: methylotrophic yeasts as factories for the production of foreign proteins, Yeast, issue.14, pp.11-1331, 1995.

F. M. Ausubel, Current protocols in molecular biology, 1987.
DOI : 10.1002/0471142727

M. S. Lantz and P. Ciborowski, [45] Zymographic techniques for detection and characterization of microbial proteases, Methods in Enzymology, vol.235, pp.563-594, 1994.
DOI : 10.1016/0076-6879(94)35171-6

P. A. Snoek-van-beurden and J. W. Von-den-hoff, Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors, BioTechniques, vol.38, issue.1, pp.73-83, 2005.
DOI : 10.2144/05381RV01

M. Soleimani, F. Mahboudi, N. Davoudi, A. Amanzadeh, M. Azizi et al., Expression of human tissue plasminogen activator in the trypanosomatid protozoan Leishmania tarentolae, Biotechnology and Applied Biochemistry, pp.48-55, 2007.

H. P. Sorensen and K. K. Mortensen, Advanced genetic strategies for recombinant protein expression in Escherichia coli, Journal of Biotechnology, vol.115, issue.2, pp.113-128, 2005.
DOI : 10.1016/j.jbiotec.2004.08.004

L. F. Vallejo and U. Rinas, Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins, Microbial Cell Factories, vol.3, issue.1, p.11, 2004.
DOI : 10.1186/1475-2859-3-11

B. Gasser, M. Saloheimo, U. Rinas, M. Dragosits, E. Rodriguez-carmona et al., Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview, Microbial Cell Factories, vol.7, issue.1, 2008.
DOI : 10.1186/1475-2859-7-11

D. B. Baruah, R. N. Dash, M. R. Chaudhari, and S. S. Kadam, Plasminogen activators: A comparison, Vascular Pharmacology, vol.44, issue.1, pp.1-9, 2006.
DOI : 10.1016/j.vph.2005.09.003

A. Upshall, A. A. Kumar, M. C. Bailey, M. D. Parker, K. P. Favreau et al., Secretion of Active Human Tissue Plasminogen Activator from the Filamentous Fungus Aspergillus Nidulans, Bio/Technology, vol.33, issue.12, pp.1301-1304, 1987.
DOI : 10.1084/jem.148.1.223

M. G. Wiebe, A. Karandikar, G. D. Robson, A. P. Trinci, J. L. Candia et al., Production of tissue plasminogen activator (t-PA) inAspergillus niger, Biotechnology and Bioengineering, vol.15, issue.2, pp.164-174, 2001.
DOI : 10.1002/bit.1156

M. S. Oka, K. L. Fong, S. A. Carr, and R. Shebuski, Characterization and biological properties of recombinant tPA produced in Drosophila cells culture, Trends in Animal Cell Culture Technology, pp.161-166, 1990.

S. A. Rouf, M. Moo-young, and Y. Chisti, Tissue-type plasminogen activator: Characteristics, applications and production technology, Biotechnology Advances, vol.14, issue.3, pp.239-266, 1996.
DOI : 10.1016/0734-9750(96)00019-5

S. Macauley-patrick, M. L. Fazenda, B. Mcneil, and L. M. Harvey, Heterologous protein production using thePichia pastoris expression system, Yeast, vol.24, issue.864, pp.249-270, 2005.
DOI : 10.1002/yea.1208

S. Curvers, L. Linneman, T. Klauser, C. Wandrey, and R. Takors, Recombinant Protein Production with Pichia pastoris in Continuous Fermentation ??? Kinetic Analysis of Growth and Product Formation, Engineering in Life Sciences, vol.2, issue.8, pp.229-235, 2002.
DOI : 10.1002/1618-2863(20020806)2:8<229::AID-ELSC229>3.0.CO;2-9

L. Cereghino, G. P. Lin-cereghino, J. Sunga, A. J. Johnson, M. A. Lim et al., New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris, Gene, vol.263, issue.1-2, pp.159-169, 2001.
DOI : 10.1016/S0378-1119(00)00576-X

S. Brocca, C. Schmidt-dannert, M. Lotti, L. Alberghina, and R. D. Schmid, Design, total synthesis, and functional overexpression of the Candida rugosa lipl gene coding for a major industrial lipase, Protein Science, vol.11, issue.6, pp.1415-1422, 1998.
DOI : 10.1002/pro.5560070618

C. Heussen and E. B. Dowdle, Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates, Analytical Biochemistry, vol.102, issue.1, pp.196-202, 1980.
DOI : 10.1016/0003-2697(80)90338-3

N. Hosomi, J. Lucero, J. H. Heo, J. A. Koziol, B. R. Copeland et al., Rapid Differential Endogenous Plasminogen Activator Expression After Acute Middle Cerebral Artery Occlusion, Stroke, vol.32, issue.6, pp.32-1341, 2001.
DOI : 10.1161/01.STR.32.6.1341

M. J. Browne, A. W. Tyrrell, C. G. Chapman, J. E. Carey, D. M. Glover et al., Isolation of a human tissue-type plasminogen-activator genomic DNA clone and its expression in mouse L cells, Gene, vol.33, issue.3, pp.33-279, 1985.
DOI : 10.1016/0378-1119(85)90235-5

A. Jalanko, J. Pirhonen, G. Pohl, and L. Hansson, Production of human tissue-type plasminogen activator in different mammalian cell lines using an Epstein-Barr virus vector, Journal of Biotechnology, vol.15, issue.1-2, pp.155-168, 1990.
DOI : 10.1016/0168-1656(90)90058-J