Production and Purification of Rabbit's Polyclonal Antibody Against Factor VIII.

Simin Sohrabi (1) , Azim Akbarzadeh (2) , Dariush Norouzian (2) , Ali Farhangi (2) , Mehri Mortazavi (2) , Mohammad Reza Mehrabi (2) , Mohsen Chiani (2) , Zahra Saffari (2) , Soheil Ghassemi (2)

1 Payame Noor University
2 Institut Pasteur d'Iran

The attempt is made to produce recombinant factor VIII but the first step in producing such product is production and purification of rabbit's polyclonal antibody against factor VIII. The second and third steps involve monoclonal antibody and recombinant factor VIII production. Factor VIII is one of the most important coagulating factor where its deficiency leads to diseases like hemophilia type A or classic. It is an inherited disease. Previously, it was obtained through fractionation of blood plasma of blood donors. After processing, factor VIII could be used to manage such patients. Due to transfer of viral disease like hepatitis and HIV through factor VIII obtained by fractionation, high cost of production, insufficiency of the donors and the process of virus removal, thus production of factor VIII through recombinant technology can be useful and helpful. The reaction between antibody and antigen is one the most specific reaction; therefore, such reaction can be employed to identify factor VIII. Thereby, rabbits were injected several times with adjuvant-linked antigen to produce antibody. The antibody was separated from the blood sample, purified and used to identify factor VIII in the research.

Keywords: Hemophilia; Factor VIII; Polyclonal antibody; Antibody purification; Recombinant antibody; Quality control

Domains: Life Sciences [q-bio]; Other [q-bio.OT]

Simin Sohrabi, Azim Akbarzadeh, Dariush Norouzian, Ali Farhangi, Mehri Mortazavi, et al.. Production and Purification of Rabbit's Polyclonal Antibody Against Factor VIII.. Indian Journal of Clinical Biochemistry, 2011, 26 (4), pp.354-9. ⟨10.1007/s12291-011-0142-2⟩. ⟨pasteur-00768857⟩