Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Production and Purification of Rabbit's Polyclonal Antibody Against Factor VIII.

Abstract : The attempt is made to produce recombinant factor VIII but the first step in producing such product is production and purification of rabbit's polyclonal antibody against factor VIII. The second and third steps involve monoclonal antibody and recombinant factor VIII production. Factor VIII is one of the most important coagulating factor where its deficiency leads to diseases like hemophilia type A or classic. It is an inherited disease. Previously, it was obtained through fractionation of blood plasma of blood donors. After processing, factor VIII could be used to manage such patients. Due to transfer of viral disease like hepatitis and HIV through factor VIII obtained by fractionation, high cost of production, insufficiency of the donors and the process of virus removal, thus production of factor VIII through recombinant technology can be useful and helpful. The reaction between antibody and antigen is one the most specific reaction; therefore, such reaction can be employed to identify factor VIII. Thereby, rabbits were injected several times with adjuvant-linked antigen to produce antibody. The antibody was separated from the blood sample, purified and used to identify factor VIII in the research.
Document type :
Journal articles
Complete list of metadata

Cited literature [19 references]  Display  Hide  Download
Contributor : Azim Akbarzadeh Connect in order to contact the contributor
Submitted on : Wednesday, December 26, 2012 - 10:00:47 AM
Last modification on : Wednesday, October 14, 2020 - 4:10:40 AM
Long-term archiving on: : Sunday, December 18, 2016 - 10:33:43 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Simin Sohrabi, Azim Akbarzadeh, Dariush Norouzian, Ali Farhangi, Mehri Mortazavi, et al.. Production and Purification of Rabbit's Polyclonal Antibody Against Factor VIII.. Indian Journal of Clinical Biochemistry, Springer Verlag (Germany), 2011, 26 (4), pp.354-9. ⟨10.1007/s12291-011-0142-2⟩. ⟨pasteur-00768857⟩



Record views