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Journal articles
Virological surveillance of dengue in Saint Martin and Saint Barthelemy, French West Indies, using blood samples on filter paper.
Abstract : To strengthen active dengue surveillance in Saint Martin and Saint Barthélemy, two French Caribbean islands, we evaluated the epidemiological usefulness of collecting blood samples from NS1-positive dengue patients on filter paper to identify the dengue serotypes circulating in these regions during a 27-month period. This approach allowed dengue serotypes to be identified by reverse transcriptase-polymerase chain reaction in 90.1% of the total set of 666 samples analyzed and, in 95.5% of the samples collected during the acute phase of the disease. This prospective virological surveillance using blood samples absorbed onto filter paper, which were stored at 4°C and shipped at ambient temperature to a specialized laboratory for analysis, allowed us to avoid the logistic and financial costs associated with shipping frozen venous blood samples. This surveillance system offers a low-cost alternative for reinforcing dengue prevention in areas where specialized laboratories do not exist, notably by facilitating the early detection of potentially new dengue serotypes.
https://hal-riip.archives-ouvertes.fr/pasteur-00786302
Contributor : Philippe Quénel Connect in order to contact the contributor
Submitted on : Friday, February 8, 2013 - 12:48:10 PM
Last modification on : Tuesday, August 3, 2021 - 5:40:02 PM
Contributor : Philippe Quénel Connect in order to contact the contributor
Submitted on : Friday, February 8, 2013 - 12:48:10 PM
Last modification on : Tuesday, August 3, 2021 - 5:40:02 PM
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