Leishmania major: Protective capacity of DNA vaccine using amastin fused to HSV-1 VP22 and EGFP in BALB/c mice model.

Azam Bolhassani; Elham Gholami; Farnaz Zahedifard; Neda Moradin; Parto Parsi; Fatemeh Doustdari; Negar Seyed; Barbara Papadopoulou; Sima Rafati

Leishmania major: Protective capacity of DNA vaccine using amastin fused to HSV-1 VP22 and EGFP in BALB/c mice model.

Azam Bolhassani ¹ Elham Gholami ¹ Farnaz Zahedifard ¹ Neda Moradin ¹ Parto Parsi ^{1, 2} Fatemeh Doustdari ¹ Negar Seyed ¹ Barbara Papadopoulou ³ Sima Rafati ^{1, *}

* Corresponding author

1 Institut Pasteur d'Iran

2 Khatam University

3 Research Centre in Infectious Diseases, CHUL Research Centre and Department of Medical Biology, Faculty of Medicine

Abstract : An intercellular spreading strategy using herpes simplex virus type 1 (HSV-1) VP22 protein is employed to enhance DNA vaccine potency of Leishmania major amastin antigen in BALB/c mice model. We evaluated the immunogenicity and protective efficacy of plasmid DNA vaccines encoding amastin-enhanced green fluorescent protein (EGFP) and VP22-amastin-EGFP. Optimal cell-mediated immune responses were observed in BALB/c mice immunized with VP22-amastin-EGFP as assessed by cytokine gene expression analysis using real time RT-PCR. Vaccination with the VP22-amastin-EGFP fusion construct elicited significantly higher IFN-gamma response upon antigen stimulation of splenocytes from immunized mice compared to amastin as a sole antigen. Mice immunized by VP22-amastin-EGFP showed partial protection following infectious challenge with L. major, as measured by parasite load in spleens. These results suggest that the development of DNA vaccines encoding VP22 fused to a target Leishmania antigen would be a promising strategy to improve immunogenicity and DNA vaccine potency.

Keywords : DNA vaccination Leishmania Amastin Cutaneous Leishmaniasis Herpes simplex virus type1 VP22 (HSV-1 VP22) Green fluorescent protein (GFP)

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Immunology / Vaccinology

Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00786634
Contributor : Sima Rafati <>
Submitted on : Saturday, February 9, 2013 - 1:25:03 PM
Last modification on : Monday, October 8, 2018 - 5:44:06 PM

Leishmania major: Protective capacity of DNA vaccine using amastin fused to HSV-1 VP22 and EGFP in BALB/c mice model.

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Leishmania major: Protective capacity of DNA vaccine using amastin fused to HSV-1 VP22 and EGFP in BALB/c mice model.

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