Skip to Main content Skip to Navigation
Journal articles

Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies.

Abstract : Reporter genes have proved to be an excellent tool for studying disease progression. Recently, the green fluorescent protein (GFP) ability to quantitatively monitor gene expression has been demonstrated in different organisms. This report describes the use of Leishmania tarentolae (L. tarentolae) expression system (LEXSY) for high and stable levels of GFP production in different Leishmania species including L. tarentolae, L. major and L. infantum. The DNA expression cassette (pLEXSY-EGFP) was integrated into the chromosomal ssu locus of Leishmania strains through homologous recombination. Fluorescent microscopic image showed that GFP transgenes can be abundantly and stably expressed in promastigote and amastigote stages of parasites. Furthermore, flow cytometry analysis indicated a clear quantitative distinction between wild type and transgenic Leishmania strains at both promastigote and amastigote forms. Our data showed that the footpad lesions with GFP-transfected L. major are progressive over time by using fluorescence small-animal imaging system. Consequently, the utilization of stable GFP-transfected Leishmania species will be appropriate for in vitro and in vivo screening of anti-leishmanial drugs and vaccine development as well as understanding the biology of the host-parasite interactions at the cellular level.
Document type :
Journal articles
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00786977
Contributor : Sima Rafati <>
Submitted on : Monday, February 11, 2013 - 10:22:41 AM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM

Identifiers

Collections

Citation

Azam Bolhassani, Taheri Tahereh, Yasaman Taslimi, Soheila Zamanilui, Farnaz Zahedifard, et al.. Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies.. Experimental Parasitology, Elsevier, 2010, 127 (3), pp.637-45. ⟨10.1016/j.exppara.2010.12.006⟩. ⟨pasteur-00786977⟩

Share

Metrics

Record views

115