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Leishmania major: disruption of signal peptidase type I and its consequences on survival, growth and infectivity.

Abstract : Leishmania major (L. major) signal peptidase type I (SPase I) is an endopeptidase encoded by a single-copy gene. In all organisms, SPase I is responsible for removing the signal peptide from secretory pre-proteins and releasing mature proteins to cellular or extra-cellular space. In this study, the role of SPase I in L. major is investigated by gene deletion using homologous recombination (HR). The null mutant of SPase I was not possible to create, suggesting that SPase I is an essential gene for parasite survival. The obtained heterozygote mutant by disrupting one allele of SPase I in L. major showed significantly reduced level of infectivity in bone marrow-derived macrophages. In addition, the heterozygote mutants are unable to cause cutaneous lesion in susceptible BALB/c mice. This is the first report showing that SPase I may have an important role in Leishmania infectivity, e.g. in differentiation and survival of amastigotes. Apparently, the SPase I expression is not essential for in vitro growth of the parasite.
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Submitted on : Monday, February 11, 2013 - 10:54:17 AM
Last modification on : Wednesday, October 14, 2020 - 4:10:28 AM

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Taheri Tahereh, Salmanian Ali-Hatef, Elham Gholami, Fatemeh Doustdari, Farnaz Zahedifard, et al.. Leishmania major: disruption of signal peptidase type I and its consequences on survival, growth and infectivity.. Experimental Parasitology, Elsevier, 2010, 126 (2), pp.135-45. ⟨10.1016/j.exppara.2010.04.009⟩. ⟨pasteur-00787030⟩

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