Utility and diagnostic performance of Mycobacterium tuberculosis complex by two immunochromatographic assays as compared with the molecular Genotype assay in Nigeria. - Archive ouverte HAL Access content directly
Journal Articles International Journal of Mycobacteriology Year : 2013

Utility and diagnostic performance of Mycobacterium tuberculosis complex by two immunochromatographic assays as compared with the molecular Genotype assay in Nigeria.

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Abstract

Among the disadvantages of smear microscopy for detection of tuberculosis cases is its inability to differentiate between Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM). This study evaluated two, new immunochromatographic assays - Capilia TB-Neo and SD Bioline - on unheated and heated cultures at 80 C for 30 min respectively for their ability to discriminate between MTB complex and NTM as compared with the molecular Genotype assay. Mycobacteria used in the study were obtained from smear-positive specimens collected from patients at four major hospitals in Cross River State, Nigeria. Capilia TB-Neo and SD Bioline showed sensitivities of 98.8% and 93.8% respectively and 100% specificity for both assays. Heating the isolates did not significantly impact the test performance. Both tests are recommended for use in rapid differentiation of strains isolated in Nigeria.

Dates and versions

pasteur-00815569 , version 1 (19-04-2013)

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Pokam Benjamin Thumamo, Anne E. Asuquo, Khye Seng Goh, Lydia N. Abia-Bassey, Nalin Rastogi. Utility and diagnostic performance of Mycobacterium tuberculosis complex by two immunochromatographic assays as compared with the molecular Genotype assay in Nigeria.. International Journal of Mycobacteriology, 2013, 2 (1), pp.34-37. ⟨10.1016/j.ijmyco.2012.12.002⟩. ⟨pasteur-00815569⟩
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