Skip to Main content Skip to Navigation
Journal articles

Screening of in vitro bioactivities of a soy protein hydrolysate separated by hollow fiber and spiral-wound ultrafiltration membranes

Abstract : The aims of this work were 1) to determine the bioactivities resulting from the hydrolysis of soy (Glycine max) proteins isolate (SPI) by a double enzymatic treatment of pepsin and pancreatin and 2) to investigate whether fractionation of the protein hydrolysates by two ultrafiltration techniques using either hollow fiber (HF) or spiral-wounded (SW) membrane (10 kDa cut-off), increases biological activity of the recovered peptides. The focus was on the screening of bioactivity of the peptide fractions using multiple in vitro bioassays such as the proliferation of cancer cells, destruction of murine norovirus-1 (MNV-1), anti-oxidative and immuno-modulating properties, and finally glucose metabolism in a muscle cell line. Significant biological effects of the peptides for both antioxidant capacity (ORAC assay) and metabolism (muscle glucose uptake assay) were found. These results show that ultrafiltration of SPI hydrolysates is a convenient process for the recovery of large amount of bioactive peptides.
Document type :
Journal articles
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00817510
Contributor : Charles M. Dozois <>
Submitted on : Wednesday, April 24, 2013 - 5:14:29 PM
Last modification on : Monday, July 20, 2020 - 1:06:04 PM

Identifiers

Collections

Citation

Cyril Roblet, Jean Amiot, Charles Lavigne, André Marette, Martin Lessard, et al.. Screening of in vitro bioactivities of a soy protein hydrolysate separated by hollow fiber and spiral-wound ultrafiltration membranes. Food Research International, Elsevier, 2012, 46 (1), pp.237-249. ⟨10.1016/j.foodres.2011.11.014⟩. ⟨pasteur-00817510⟩

Share

Metrics

Record views

169