Stability of reference proteins in human placenta: general protein stains are the benchmark. - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue Placenta Année : 2012

Stability of reference proteins in human placenta: general protein stains are the benchmark.

Résumé

The stability of reference proteins in semi-quantitative Western blot experiments in normal and diseased placenta has never been studied. This study aims to determine the stability of five reference proteins and two general protein stains in placentas from preeclampsia, gestational diabetes mellitus and matched control pregnancies. The stability of the reference proteins was analysed using indicators of inter-group (P value) and intra-group (coefficient of variation) stability. The effect of different normalization strategies was determined by normalizing serotonin transporter (SERT) expression against the different reference protein markers. Results show significant expression variability of β-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylprolyl isomerase A (PPIA) and α-tubulin, and that amido black staining is the most stable reference protein marker. Furthermore, results show that SERT expression significantly differs according to the reference protein markers used for its normalization. The present study demonstrated the importance of using stable reference protein markers and normalization strategy in order to get correct results in semi-quantitative Western blot experiments in placental tissues.

Domaines

Toxicologie

Dates et versions

pasteur-00818357 , version 1 (26-04-2013)

Identifiants

Citer

D. Lanoix, J. St-Pierre, A. A. Lacasse, M. Viau, J. Lafond, et al.. Stability of reference proteins in human placenta: general protein stains are the benchmark.. Placenta, 2012, 33 (3), pp.151-6. ⟨10.1016/j.placenta.2011.12.008⟩. ⟨pasteur-00818357⟩

Collections

RIIP INRS-IAF
30 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More