Skip to Main content Skip to Navigation
Journal articles

Stability of reference proteins in human placenta: general protein stains are the benchmark.

Abstract : The stability of reference proteins in semi-quantitative Western blot experiments in normal and diseased placenta has never been studied. This study aims to determine the stability of five reference proteins and two general protein stains in placentas from preeclampsia, gestational diabetes mellitus and matched control pregnancies. The stability of the reference proteins was analysed using indicators of inter-group (P value) and intra-group (coefficient of variation) stability. The effect of different normalization strategies was determined by normalizing serotonin transporter (SERT) expression against the different reference protein markers. Results show significant expression variability of β-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylprolyl isomerase A (PPIA) and α-tubulin, and that amido black staining is the most stable reference protein marker. Furthermore, results show that SERT expression significantly differs according to the reference protein markers used for its normalization. The present study demonstrated the importance of using stable reference protein markers and normalization strategy in order to get correct results in semi-quantitative Western blot experiments in placental tissues.
Document type :
Journal articles
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00818357
Contributor : Charles M. Dozois <>
Submitted on : Friday, April 26, 2013 - 4:41:02 PM
Last modification on : Friday, March 27, 2020 - 3:18:03 PM

Identifiers

Collections

Citation

D. Lanoix, J. St-Pierre, A. A. Lacasse, M. Viau, J. Lafond, et al.. Stability of reference proteins in human placenta: general protein stains are the benchmark.. Placenta, Elsevier, 2012, 33 (3), pp.151-6. ⟨10.1016/j.placenta.2011.12.008⟩. ⟨pasteur-00818357⟩

Share

Metrics

Record views

223