Liquid chromatography/mass spectrometry for the detection and quantification of N-acyl-L-homoserine lactones and 4-hydroxy-2-alkylquinolines.

François Lépine (1) , Eric Déziel (1)

1 INRS-IAF - Institut Armand Frappier

High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-L-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.

Keywords: Bacteria; Quorum sensing; Acyl homoserine lactone; 4-Hydroxy-2-alkylquinoline; Analysis; Quantification; Mass spectrometry; Liquid chromatography

Domains: Biotechnology; Biotechnology

François Lépine, Eric Déziel. Liquid chromatography/mass spectrometry for the detection and quantification of N-acyl-L-homoserine lactones and 4-hydroxy-2-alkylquinolines.. Methods in Molecular Biology, 2011, 692, pp.61-9. ⟨10.1007/978-1-60761-971-0_5⟩. ⟨pasteur-00819401⟩