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Canine distemper viruses expressing a hemagglutinin without N-glycans lose virulence but retain immunosuppression.

Abstract : Paramyxovirus glycoproteins are posttranslationally modified by the addition of N-linked glycans, which are often necessary for correct folding, processing, and cell surface expression. To establish the contribution of N glycosylation to morbillivirus attachment (H) protein function and overall virulence, we first determined the use of the potential N-glycosylation sites in the canine distemper virus (CDV) H proteins. Biochemical characterization revealed that the three sites conserved in all strains were N glycosylated, whereas only two of the up to five additional sites present in wild-type strains are used. A wild-type virus with an H protein reproducing the vaccine strain N-glycosylation pattern remained lethal in ferrets but with a prolonged course of disease. In contrast, introduction of the vaccine H protein in the wild-type context resulted in complete attenuation. To further characterize the role of N glycosylation in CDV pathogenesis, the N-glycosylation sites of wild-type H proteins were successively deleted, including a nonstandard site, to ultimately generate a nonglycosylated H protein. Despite reduced expression levels, this protein remained fully functional. Recombinant viruses expressing N-glycan-deficient H proteins no longer caused disease, even though their immunosuppressive capacities were retained, indicating that reduced N glycosylation contributes to attenuation without affecting immunosuppression.
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Contributor : Charles M. Dozois Connect in order to contact the contributor
Submitted on : Wednesday, May 1, 2013 - 9:07:37 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM

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Bevan Sawatsky, Veronika von Messling. Canine distemper viruses expressing a hemagglutinin without N-glycans lose virulence but retain immunosuppression.. Journal of Virology, 2010, 84 (6), pp.2753-61. ⟨10.1128/JVI.01813-09⟩. ⟨pasteur-00819541⟩



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