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Journal articles
Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356.
Abstract : cis-Biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (BphB) is involved in the aerobic biodegradation of biphenyl and polychlorinated biphenyls. BphB from Pandoraea pnomenusa strain B-356 was overexpressed in Escherichia coli, purified to homogeneity and crystallized. Crystals were obtained by the sitting-drop vapour-diffusion method using polyethylene glycol 3350 and 0.2 M sodium malonate. A BphB crystal diffracted to 2.8 Å resolution and belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 75.2, c = 180.4 Å. Preliminary crystallographic analysis indicated the presence of two molecules in the asymmetric unit, giving a Matthews coefficient of 2.2 Å(3) Da(-1) and a solvent content of 44%.
https://hal-riip.archives-ouvertes.fr/pasteur-00819542
Contributor : Charles M. Dozois <>
Submitted on : Wednesday, May 1, 2013 - 9:09:44 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM
Contributor : Charles M. Dozois <>
Submitted on : Wednesday, May 1, 2013 - 9:09:44 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM
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