Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356.

? Sakshi 1 Dipak N Patil 1 Shailly Tomar 1 Michel Sylvestre 2 Pravindra Kumar 1, *
* Corresponding author
1 Department of Biotechnology [Roorkee)
2 INRS-IAF - Institut Armand Frappier
Abstract : cis-Biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (BphB) is involved in the aerobic biodegradation of biphenyl and polychlorinated biphenyls. BphB from Pandoraea pnomenusa strain B-356 was overexpressed in Escherichia coli, purified to homogeneity and crystallized. Crystals were obtained by the sitting-drop vapour-diffusion method using polyethylene glycol 3350 and 0.2 M sodium malonate. A BphB crystal diffracted to 2.8 Å resolution and belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 75.2, c = 180.4 Å. Preliminary crystallographic analysis indicated the presence of two molecules in the asymmetric unit, giving a Matthews coefficient of 2.2 Å(3) Da(-1) and a solvent content of 44%.
keyword : cis-biphenyl-2 3-dihydrodiol-2 3-dehydrogenase Pandoraea pnomenusa biodegradation
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Submitted on : Wednesday, May 1, 2013 - 9:09:44 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM

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? Sakshi, Dipak N Patil, Shailly Tomar, Michel Sylvestre, Pravindra Kumar. Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356.. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2010, 66 (Pt 11), pp.1517-20. ⟨10.1107/S1744309110036894⟩. ⟨pasteur-00819542⟩

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