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Adaptation of the highly productive T7 expression system to Streptomyces lividans.

Abstract : Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.
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Submitted on : Wednesday, May 1, 2013 - 9:32:20 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM

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François-Xavier Lussier, François Denis, François Shareck. Adaptation of the highly productive T7 expression system to Streptomyces lividans.. Applied and Environmental Microbiology, American Society for Microbiology, 2010, 76 (3), pp.967-70. ⟨10.1128/AEM.02186-09⟩. ⟨pasteur-00819554⟩

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