Adaptation of the highly productive T7 expression system to Streptomyces lividans.

François-Xavier Lussier; François Denis; François Shareck

doi:10.1128/AEM.02186-09

Journal articles

Adaptation of the highly productive T7 expression system to Streptomyces lividans.

François-Xavier Lussier ^{1, *} François Denis ¹ François Shareck ¹

* Corresponding author

1 INRS-IAF - Institut Armand Frappier

Abstract : Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.

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Journal articles

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Life Sciences [q-bio] / Biotechnology

Computer Science [cs] / Biotechnology

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https://hal-riip.archives-ouvertes.fr/pasteur-00819554
Contributor : Charles M. Dozois <>
Submitted on : Wednesday, May 1, 2013 - 9:32:20 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM

Adaptation of the highly productive T7 expression system to Streptomyces lividans.

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