Excretion of norsteroids' phase II metabolites of different origin in human.

Claudiane Guay (1) , Danielle Goudreault (1) , Wilhem Schänzer (2) , Ulrich Flenker (2) , Christiane Ayotte (1)

1 INRS-IAF - Institut Armand Frappier
2 Institute of Biochemistry

The urinary phase II metabolites of norsteroids, 19-norandrosterone, 19-noretiocholanolone and 19-norepiandrosterone glucuronide and sulphate, were analyzed in samples collected during the pregnancy, following the administration of norsteroids or the consumption of edible parts of non-castrated pig and in athletes' samples in which they were found during routine controls. The level of the sulfo- and glucuroconjugated metabolites was precisely determined by GC/HRMS, after selective hydrolysis. The goal was to evaluate whether the fine analysis of the norsteroid conjugates produced and excreted in different conditions would show a pattern that could be linked to their origin. The delta (13)C values of the metabolites formed following the ingestion of edible parts of non-castrated pig were measured by isotope ratio mass spectrometry. Our results indicated that it is not possible to determine the origin of the urinary metabolites based upon the sole evaluation of the different metabolites and conjugates. The GC/C/IRMS is the only method permitting to distinguish between the exogenous and endogenous origin of the metabolites.

Domaines: Toxicologie

Claudiane Guay, Danielle Goudreault, Wilhem Schänzer, Ulrich Flenker, Christiane Ayotte. Excretion of norsteroids' phase II metabolites of different origin in human.. Steroids, 2009, 74 (3), pp.350-8. ⟨10.1016/j.steroids.2008.10.015⟩. ⟨pasteur-00819930⟩