Increased galectin-7 gene expression in lymphoma cells is under the control of DNA methylation.

Abstract : Recent studies have reported that elevated levels of galectin-7 in different types of cancer. The mechanisms underlying its abnormal regulation in cancer cells remain, however, unknown. Here, we have examined the relationship between galectin-7 and p53, a gene previously associated with upregulation of galectin-7. While RNA and protein analyses revealed a consistent and irreversible upregulation of galectin-7 throughout progression of lymphoma, no correlation with p53 was found. In fact, most of the lymphoma cell lines expressing high levels of galectin-7 did not express any detectable level of p53, although expressed p21(Waf1/Cip1) gene following doxorubicin treatment, indicating that p53 was functional in these cells. Methylation-specific polymerase chain reaction (MS-PCR) analyses rather suggested that galectin-7 expression was associated with changes in DNA methylation. This conclusion was supported by data using demethylating agent 5-aza-dC. Furthermore, disruption of the DNA methylases dnmt1 and dnmt3a induced galectin-7. Collectively, our data suggest that abnormal expression of galectin-7 in lymphoma cells is not dependent on p53, but is rather associated with DNA hypomethylation.
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Submitted on : Thursday, May 2, 2013 - 8:17:35 PM
Last modification on : Tuesday, August 27, 2019 - 1:00:56 AM

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Mélanie Demers, Julie Couillard, Giuseppina Giglia-Mari, Thierry Magnaldo, Yves St-Pierre. Increased galectin-7 gene expression in lymphoma cells is under the control of DNA methylation.. Biochemical and Biophysical Research Communications, Elsevier, 2009, 387 (3), pp.425-9. ⟨10.1016/j.bbrc.2009.07.015⟩. ⟨pasteur-00819941⟩

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