Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages.

Abstract : AIMS: To evaluate the immunosuppressive properties of the exopolysaccharide (EPS) from high-EPS producer Lactobacillus rhamnosus RW-9595M on inflammatory cytokines produced by macrophages. METHODS AND RESULTS: The conditioned media (CM) were produced by macrophages treated with parental Lact. rhamnosus ATCC 9595 and its isogenic variant, the high-EPS producer Lact. rhamnosus RW-9595M, and the levels of TNF-alpha, IL-6, IL-10 and IL-12 were evaluated. Results revealed that CM from parental Lact. rhamnosus induced higher levels of TNF-alpha, IL-6 and IL-12 but inhibited IL-10 production, whereas its mucous variant induced low or no TNF-alpha and IL-6. Addition of purified EPS to macrophages treated with parental Lact. rhamnosus decreased the inflammatory cytokines and inhibited the metabolic activity of lymphocytes. The intermediate polysaccharide chains (16-30 units) produced by time-controlled hydrolysis of EPS increased the IL-10 produced by macrophages. CONCLUSIONS: Polysaccharide chains of EPS induced immunosuppression by the production of macrophagic anti-inflammatory IL-10. SIGNIFICANCE AND IMPACT OF THE STUDY: These results indicate that the EPS from Lact. rhamnosus RW-9595M may be useful as a new immunosuppressive product in dairy food.
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Submitted on : Thursday, May 2, 2013 - 9:04:28 PM
Last modification on : Wednesday, September 11, 2019 - 1:50:05 PM

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C. Bleau, A. Monges, K. Rashidan, J.-P. Laverdure, M. Lacroix, et al.. Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages.. Journal of Applied Microbiology, Wiley, 2010, 108 (2), pp.666-75. ⟨10.1111/j.1365-2672.2009.04450.x⟩. ⟨pasteur-00819959⟩

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