Correlation of serum anti-Müllerian hormone with accelerated follicle loss following 4-vinylcyclohexene diepoxide-induced follicle loss in mice.

Sukhdeep K Sahambi 1 Jenny A Visser 2 Axel P N Themmen 2 Loretta P Mayer 3 Patrick J Devine 1, *
* Corresponding author
1 INRS-IAF - Institut Armand Frappier
2 Department of Internal Medicine
3 Department of Biological Sciences [Flagstaff]
Abstract : A chemically induced model of ovarian failure has been developed in rodents, and was used to test whether or not anti-Müllerian hormone (AMH) can be used as a non-invasive measure of primordial follicle numbers. Repeated exposures of mice to 4-vinylcyclohexene diepoxide (VCD) induce loss of primordial and earliest growing ovarian follicles. An accelerated exposure regimen was used to eliminate small ovarian follicles in C57BL6/J mice (240mg VCD/kg/day, 5 days, i.p.). Follicle populations were determined and correlated with circulating AMH levels. Exposures decreased only primordial and small primary follicles by 96% on day 16 after initiating exposures, followed by almost complete follicle elimination on days 37-100. AMH levels in VCD-exposed mice were similar to vehicle-treated mice on day 16, but became significantly lower or undetectable at later time points. Thus, AMH correlated well with growing follicle numbers. AMH only correlated with primordial follicles at time points after ovarian insult at which their loss led to decreased growing follicle numbers.
Document type :
Journal articles
Complete list of metadatas
https://hal-riip.archives-ouvertes.fr/pasteur-00819969
Contributor : Charles M. Dozois <>
Submitted on : Thursday, May 2, 2013 - 9:24:37 PM
Last modification on : Thursday, February 7, 2019 - 2:20:28 PM

Identifiers

Collections

RIIP | INRS-IAF

Citation

Sukhdeep K Sahambi, Jenny A Visser, Axel P N Themmen, Loretta P Mayer, Patrick J Devine. Correlation of serum anti-Müllerian hormone with accelerated follicle loss following 4-vinylcyclohexene diepoxide-induced follicle loss in mice.. Reproductive Toxicology, Elsevier, 2008, 26 (2), pp.116-22. ⟨10.1016/j.reprotox.2008.07.005⟩. ⟨pasteur-00819969⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

75

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD