Adjuvant and immunogenic activities of the 73kDa N-terminal alpha-domain of BrkA autotransporter and Cpn60/60kDa chaperonin of Bordetella pertussis.

Vera C B Cainelli Gebara (1) , Luciana Risoléo (1) , Alexandre P y Lopes (1) , Vera R F Ferreira (1) , Wagner Quintilio (2) , François Lépine (3) , Wilmar D Silva (4) , Isaías Raw (1)

1 Centro de Biotecnologia [Sao Paulo]
2 Serviço de Controle de Qualidade
3 INRS-IAF - Institut Armand Frappier
4 Centro de Biociencias e Biotecnologia

A soluble fraction obtained from Bordetella pertussis was evaluated as adjuvant for the pertussis component of the Diphtheria-Pertussis-Tetanus (DPT) vaccine. High levels of antibodies were induced, and a 78% protection rate of mice challenged with live B. pertussis was observed. Two proteins were identified as the 73 kDa N-terminal alpha-domain of BrkA autotransporter protein and the Cpn60/60 kDa chaperonin. Both stimulated antibodies against pertussis and induced a 42% protection rate against the challenge. IgG1 and IgG2a were stimulated suggesting that the immune response could be modulated to produce Th1 or Th2.

Domains: Life Sciences [q-bio]; Biotechnology; Computer Science [cs]; Biotechnology

Vera C B Cainelli Gebara, Luciana Risoléo, Alexandre P y Lopes, Vera R F Ferreira, Wagner Quintilio, et al.. Adjuvant and immunogenic activities of the 73kDa N-terminal alpha-domain of BrkA autotransporter and Cpn60/60kDa chaperonin of Bordetella pertussis.. Vaccine, 2007, 25 (4), pp.621-9. ⟨10.1016/j.vaccine.2006.08.033⟩. ⟨pasteur-00819998⟩