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Analysis of the bacterial community inhabiting an aerobic thermophilic sequencing batch reactor (AT-SBR) treating swine waste.

P. Juteau 1, * D. Tremblay 1 R. Villemur 1 J.-G. Bisaillon 1 R. Beaudet 1 
* Corresponding author
Abstract : The microflora of a self-heating aerobic thermophilic sequencing batch reactor (AT-SBR) treating swine waste was investigated by a combination of culture and culture-independent techniques. The temperature increased quickly in the first hours of the treatment cycles and values up to 72 degrees C were reached. Denaturing gradient gel electrophoresis of the PCR-amplified V3 region of 16S rDNA (PCR-DGGE) revealed important changes in the bacterial community during 3-day cycles. A clone library was constructed with the near-full-length 16S rDNA amplified from a mixed-liquor sample taken at 60 degrees C. Among the 78 non-chimeric clones analysed, 20 species (here defined as clones showing more than 97% sequence homology) were found. In contrast to other culture-independent bacterial analyses of aerobic thermophilic wastewater treatments, species belonging to the Bacilli class were dominant (64%) with Bacillus thermocloacae being the most abundant species (38%). The other Bacilli could not be assigned to a known species. Schineria larvae was the second most abundant species (14%) in the clone library. Four species were also found among the 19 strains isolated, cultivated and identified from samples taken at 40 degrees C and 60 degrees C. Ten isolates showed high 16S rDNA sequence homology with the dominant bacterium of a composting process that had not been previously isolated.
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https://hal-riip.archives-ouvertes.fr/pasteur-00820030
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Submitted on : Friday, May 3, 2013 - 1:00:50 AM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM

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P. Juteau, D. Tremblay, R. Villemur, J.-G. Bisaillon, R. Beaudet. Analysis of the bacterial community inhabiting an aerobic thermophilic sequencing batch reactor (AT-SBR) treating swine waste.. Applied Microbiology and Biotechnology, Springer Verlag, 2004, 66 (1), pp.115-22. ⟨10.1007/s00253-004-1692-5⟩. ⟨pasteur-00820030⟩

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