Effect of the addition of CMC on the aggregation behaviour of proteins

H. Yu (1) , S.F Sabato (2) , G. d'Aprano (3) , Monique Lacroix (1)

1 INRS-IAF - Institut Armand Frappier
2 Departamento de Aplicações Nucleares
3 Rhoxalpharma

The effect of carboxymethylcellulose (CMC) on the aggregation of formulation based on calcium caseinate, commercial whey protein (WPC), and a 1:1 mixture of soy protein isolate (SPI) and whey protein isolate (WPI) was investigated. Protein aggregation could be observed upon addition of CMC, as demonstrated by size-exclusion chromatography. This aggregation behaviour was enhanced by means of physical treatments, such as heating at 90°C for 30 min or gamma-irradiation at 32 kGy. A synergy resulted from the combination of CMC to gamma-irradiation in Caseinate/CMC and SPI/WPI/CMC formulations. Furthermore, CMC prevented precipitation in irradiated protein solutions for a period of more than 3 months at 4°C.

Keywords: Carboxymethylcellulose; Proteins; Aggregation; Gamma-irradiation

Domains: Life Sciences [q-bio]; Human health and pathology; Infectious diseases

H. Yu, S.F Sabato, G. d'Aprano, Monique Lacroix. Effect of the addition of CMC on the aggregation behaviour of proteins. Radiation Physics and Chemistry, 2004, 71 (1-2), pp.131-135. ⟨10.1016/j.radphyschem.2004.04.003⟩. ⟨pasteur-00820037⟩