Generation and characterization of a functional Nanobody against the vascular endothelial growth factor receptor-2; angiogenesis cell receptor. - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue Molecular Immunology Année : 2012

Generation and characterization of a functional Nanobody against the vascular endothelial growth factor receptor-2; angiogenesis cell receptor.

Résumé

Vascular endothelial growth factor receptor-2 (VEGFR2) is an important tumor-associated receptor and blockade of the VEGF receptor signaling can lead to the inhibition of neovascularization and tumor metastasis. Nanobodies are the smallest intact antigen binding fragments derived from heavy chain-only antibodies occurring in camelids. Here, we describe the identification of a VEGFR2-specific Nanobody, named 3VGR19, from dromedaries immunized with a cell line expressing high levels of VEGFR2. We demonstrate by FACS, that 3VGR19 Nanobody specifically binds VEGFR2 on the surface of 293KDR and HUVECs cells. Furthermore, the 3VGR19 Nanobody potently inhibits formation of capillary-like structures. These data show the potential of Nanobodies for the blockade of VEGFR2 signaling and provide a basis for the development of novel cancer therapeutics.

Mots clés

Domaines

Génétique
Fichier sous embargo
Fichier sous embargo
Date de visibilité indéterminée
Loading...

Dates et versions

pasteur-00820543 , version 1 (06-05-2013)

Identifiants

Citer

Mahdi Behdani, Sirous Zeinali, Hossein Khanahmad, Morteza Karimipour, Nader Asadzadeh, et al.. Generation and characterization of a functional Nanobody against the vascular endothelial growth factor receptor-2; angiogenesis cell receptor.. Molecular Immunology, 2012, 50 (1-2), pp.35-41. ⟨10.1016/j.molimm.2011.11.013⟩. ⟨pasteur-00820543⟩

Collections

RIIP RIIP_IRAN
91 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More