J. Bustos-valenzuela, E. Halcsik, E. Bassi, M. Demasi, J. Granjeiro et al., Expression, Purification, Bioactivity, and Partial Characterization of a Recombinant Human Bone Morphogenetic Protein-7 Produced in Human 293T Cells, Molecular Biotechnology, vol.85, issue.2, pp.118-126, 2010.
DOI : 10.1007/s12033-010-9287-0

C. Kim, S. Oh, J. Rhee, E. Lee, and T. Yoon, Expression and purification of recombinant human bone morphogenetic protein-7 (rhBMP-7) in Bacillus subtilis, Biotechnology and Bioprocess Engineering, vol.25, issue.5, pp.830-836, 2010.
DOI : 10.1007/s12257-009-3116-y

D. Lee, H. Baek, M. Lee, and J. Park, Production of bone morphogenetic protein-7 using pET expression system, Current Applied Physics, vol.5, issue.5, pp.422-425, 2005.
DOI : 10.1016/j.cap.2005.01.003

L. Chen, Y. Hung, and Y. Zhang, Expression and secretion of human bone morphogenetic protein-7 in pichia pastoris, Chinese Journal of Biotechnology, vol.22, issue.6, pp.907-914, 2006.
DOI : 10.1016/S1872-2075(06)60064-2

H. Dinopoulos and P. Giannoudis, (iv) The use of bone morphogenetic proteins (BMPs) in long-bone non-unions, Current Orthopaedics, vol.21, issue.4, pp.268-279, 2007.
DOI : 10.1016/j.cuor.2007.07.007

M. Alaoui-ismaili and D. Falb, Design of second generation therapeutic recombinant bone morphogenetic proteins, Cytokine & Growth Factor Reviews, vol.20, issue.5-6, pp.5-6501, 2009.
DOI : 10.1016/j.cytogfr.2009.10.001

D. Han, W. Liu, Q. Ao, and G. Wang, Optimal delivery systems for bone morphogenetic proteins in orthopedic applications should model initial tissue repair structures by using a heparin-incorporated fibrin???fibronectin matrix, Medical Hypotheses, vol.71, issue.3, pp.374-378, 2008.
DOI : 10.1016/j.mehy.2008.01.035

N. Kübler, K. Würzler, J. Reuther, E. Sieber, T. Kirchner et al., The influence of various factors on the bone forming properties of recombinant BMPs, Mund-, Kiefer- und Gesichtschirurgie, vol.4, issue.S2, pp.465-469, 2000.
DOI : 10.1007/PL00012693

T. Takada, T. Katagiri, M. Ifuku, N. Morimura, M. Kobayashi et al., Sulfated Polysaccharides Enhance the Biological Activities of Bone Morphogenetic Proteins, Journal of Biological Chemistry, vol.278, issue.44, pp.43229-43235, 2003.
DOI : 10.1074/jbc.M300937200

A. Irie, M. Takami, H. Kubo, N. Sekino-suzuki, K. Kasahara et al., Heparin enhances osteoclastic bone resorption by inhibiting osteoprotegerin activity, Bone, vol.41, issue.2, pp.165-174, 2007.
DOI : 10.1016/j.bone.2007.04.190

Z. Johnson, A. Proudfoot, and T. Handel, Interaction of chemokines and glycosaminoglycans: A new twist in the regulation of chemokine function with opportunities for therapeutic intervention, Cytokine & Growth Factor Reviews, vol.16, issue.6, pp.625-636, 2005.
DOI : 10.1016/j.cytogfr.2005.04.006

K. Würzler, J. Emmert, F. Eichelsbacher, N. Kübler, W. Sebald et al., Evaluation of osteoinductive potency of genetically modified BMP-2 variations, Mund Kiefer Gesichtschir, vol.8, issue.2, pp.83-92, 2004.

R. Depprich, J. Handschel, W. Sebald, N. Kübler, and K. Würzler, Comparison of the osteogenic activity of bone morphogenetic protein (BMP) mutants, Mund-, Kiefer- und Gesichtschirurgie, vol.43, issue.6, pp.363-368, 2005.
DOI : 10.1007/s10006-005-0644-2

J. Robbens, D. Coen, W. Fiers, W. Remaut, and E. , Improved periplasmic production of biologically active murine interleukin-2 in Escherichia coli through a single amino acid change at the cleavage site, Process Biochemistry, vol.41, issue.6, pp.1343-1346, 2006.
DOI : 10.1016/j.procbio.2006.01.009

C. Soares, F. Gomide, E. Ueda, and P. Bartolini, Periplasmic expression of human growth hormone via plasmid vectors containing the ??PL promoter: use of HPLC for product quantification, Protein Engineering Design and Selection, vol.16, issue.12, pp.1131-1138, 2003.
DOI : 10.1093/protein/gzg114

B. Hernández, V. , P. Maldonado, L. , M. Rivero et al., Periplasmic expression and recovery of human interferon gamma in Escherichia coli, Protein Expression and Purification, vol.59, issue.1, pp.169-174, 2008.
DOI : 10.1016/j.pep.2008.01.019

A. Ambrus, B. Torocsik, and V. Adam-vizi, Periplasmic cold expression and one-step purification of human dihydrolipoamide dehydrogenase, Protein Expression and Purification, vol.63, issue.1, pp.50-57, 2009.
DOI : 10.1016/j.pep.2008.09.009

R. Jazii, F. Karkhane, A. Yakhchali, B. Fatemi, S. Deezagi et al., A simplified purification procedure for recombinant human granulocyte macrophage-colony stimulating factor from periplasmic space of Escherichia coli, Journal of Chromatography B, vol.856, issue.1-2, pp.214-221, 2007.
DOI : 10.1016/j.jchromb.2007.06.013

N. Nossal and L. Heppel, The release of enzymes by osmotic shock from Escherichia coli in exponential phase, J Biol Chem, vol.241, issue.13, pp.3055-3062, 1966.

U. Laemmli, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature, vol.244, issue.5259, pp.680-685, 1970.
DOI : 10.1038/227680a0

O. Lowry, N. Rosebrough, A. Farr, and R. Randall, Protein measurement with the Folin phenol reagent, J Biol Chem, vol.193, issue.1, pp.265-275, 1951.

D. Mahoney, J. Whittle, C. Milner, S. Clark, B. Mulloy et al., A method for the non-covalent immobilization of heparin to surfaces, Analytical Biochemistry, vol.330, issue.1, pp.123-129, 2004.
DOI : 10.1016/j.ab.2004.03.055

M. Fisher, Y. Li, M. Seghatoleslami, C. Dealy, and R. Kosher, Heparan sulfate proteoglycans including syndecan-3 modulate BMP activity during limb cartilage differentiation, Matrix Biology, vol.25, issue.1, pp.27-39, 2006.
DOI : 10.1016/j.matbio.2005.07.008

J. Granjeiro, R. Oliveira, J. Bustos-valenzuela, M. Sogayar, and R. Taga, Bone morphogenetic proteins: from structure to clinical use, Brazilian Journal of Medical and Biological Research, vol.38, issue.10, pp.1463-1473, 2005.
DOI : 10.1590/S0100-879X2005001000003

E. Carlisle and J. Fischgrund, Bone morphogenetic proteins for spinal fusion, The Spine Journal, vol.5, issue.6, pp.240-249, 2005.
DOI : 10.1016/j.spinee.2005.02.014

R. Ruppert, E. Hoffmann, and W. Sebald, Human Bone Morphogenetic Protein 2 Contains a Heparin-Binding Site which Modifies Its Biological Activity, European Journal of Biochemistry, vol.74, issue.1, pp.295-302, 1996.
DOI : 10.1006/bbrc.1994.2898

T. Sampath, J. Maliakal, P. Hauschka, W. Jones, H. Sasak et al., Recombinant human osteogenic protein-1 (hOP-1) induces new bone formation in vivo with a specific activity comparable with natural bovine osteogenic protein and stimulates osteoblast proliferation and differentiation in vitro, J Biol Chem, vol.267, issue.28, pp.20352-20362, 1992.

D. Lee, H. Suh, D. Han, B. Park, J. Lee et al., The Effects of Recombinant Human BMP-7, Prepared from a COS-7 Expression System, on the Proliferation and Differentiation of Rat Newborn Calvarial Osteoblasts, Yonsei Medical Journal, vol.44, issue.4, pp.593-601, 2003.
DOI : 10.3349/ymj.2003.44.4.593

B. Swencki-underwood, J. Mills, J. Vennarini, K. Boakye, J. Luo et al., Expression and characterization of a human BMP-7 variant with improved biochemical properties, Protein Expression and Purification, vol.57, issue.2, pp.312-319, 2008.
DOI : 10.1016/j.pep.2007.09.016

L. Vallejo, M. Brokelmann, S. Marten, S. Trappe, J. Cabrera-crespo et al., Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli, Journal of Biotechnology, vol.94, issue.2, pp.185-194, 2002.
DOI : 10.1016/S0168-1656(01)00425-4

C. Kirker-head, Potential applications and delivery strategies for bone morphogenetic proteins, Advanced Drug Delivery Reviews, vol.43, issue.1, pp.65-92, 2000.
DOI : 10.1016/S0169-409X(00)00078-8

J. Kiany, A. Zomorodipour, M. Raji, and M. Sanati, Construction of recombinant plasmids for periplasmic expression of human growth hormone in Escherichia coli under T7 and lac promoters, J Sci IR Iran, vol.14, issue.4, pp.311-316, 2003.