Skip to Main content Skip to Navigation
New interface
Journal articles

Characterization of immune responses induced by combined Clade-A HIV-1 recombinant Adenovectors in mice.

Abstract : Background: Numerous evidences indicate that in some HIV-1 positive patients, the humoral and cellular immune responses are induced against HIV-1 proteins and this is inversely related to the progress of infection. Objective: The aim of this study was the evaluation of the Adenovectors containing HIV genes in induction of immune responses in mice. Methods: The HIV-1 genes including gag p24, rev, nef and exon-1 of tat were amplified from HIV-1 RNA (clade-A). The cDNA of each gene was cloned into a transfer vector. The transfer vector was then co-transformed into E. coli strain BJ5183 together with pAdenovector ∆E1/E3. The recombinant adenoviral construct was transfected into QBI-293A cells. Recombinant viruses were purified and titrated on 293 cell plates. Expression of transgenes was evaluated using western blotting. Then 10E12 viral particles were injected into 15 groups of 5 mice and all patterns of combination of these 4 HIV-1 genes were evaluated. After 2 weeks, humoral and cellular immune responses were evaluated using ELISA, cell proliferation and ELISpot (IL-2, IL-4 and IFN-γ) assays, consecutively. Results: It was demonstrated that each gene was expressed. The response targets were mostly toward Th1, though several Th2 responses were also observed. Single injection in our study induced a good cellular response but the humoral responses were not as strong as the cellular ones. Conclusion: Considering and comparing all results and evaluating the various possible interactions revealed that simultaneous injection of tat and gag has enhanced the humoral and cellular responses.
Document type :
Journal articles
Complete list of metadata

Cited literature [39 references]  Display  Hide  Download
Contributor : Morteza Karimipour Connect in order to contact the contributor
Submitted on : Saturday, June 1, 2013 - 9:37:14 AM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM
Long-term archiving on: : Tuesday, April 4, 2017 - 2:52:17 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document


  • HAL Id : pasteur-00829014, version 1
  • PUBMED : 20876987



Saeed Bayanolhagh, Mahtab Alinezhad, Koorosh Kamali, Maryam Foroughi, Hamid Reza Khorram Khorshid, et al.. Characterization of immune responses induced by combined Clade-A HIV-1 recombinant Adenovectors in mice.. Iranian Journal of Immunology, 2010, 7 (3), pp.162-76. ⟨pasteur-00829014⟩



Record views