Activation of minority-variant Plasmodium vivax hypnozoites following artesunate + amodiaquine treatment in a 23-year old man with relapsing malaria in Antananarivo, Madagascar.

Abstract : In endemic areas, Plasmodium vivax relapses are difficult to distinguish from new infections. Genotyping of patients who experience relapse after returning to a malaria-free area can be used to explore the nature of hypnozoite activation and relapse. This paper describes a person who developed P. vivax malaria for the first time after travelling to Boriziny in the malaria endemic coastal area of Madagascar, then suffered two P. vivax relapses 11 weeks and 21 weeks later despite remaining in Antananarivo in the malaria-free central highlands area. He was treated with the combination artesunate + amodiaquine according to the national malaria policy in Madagascar. Genotyping by PCR-RFLP at pvmsp-3alpha as well as pvmsp1 heteroduplex tracking assay (HTA) showed the same dominant genotype at each relapse. Multiple recurring minority variants were also detected at each relapse, highlighting the propensity for multiple hypnozoite clones to activate simultaneously to cause relapse.
Document type :
Journal articles
Complete list of metadatas

Cited literature [14 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00835064
Contributor : Princy Rakotoarison <>
Submitted on : Tuesday, June 18, 2013 - 8:33:03 AM
Last modification on : Wednesday, August 21, 2019 - 11:22:20 AM
Long-term archiving on : Thursday, September 19, 2013 - 4:08:21 AM

File

23721298AndrianaranjakaVMalarJ...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Voahangy Andrianaranjaka, Jessica T Lin, Christopher Golden, Jonathan J Juliano, Milijaona Randrianarivelojosia. Activation of minority-variant Plasmodium vivax hypnozoites following artesunate + amodiaquine treatment in a 23-year old man with relapsing malaria in Antananarivo, Madagascar.. Malaria Journal, BioMed Central, 2013, 12 (1), pp.177. ⟨10.1186/1475-2875-12-177⟩. ⟨pasteur-00835064⟩

Share

Metrics

Record views

154

Files downloads

147