Skip to Main content Skip to Navigation
Journal articles

Mono- and Dithiol Glutaredoxins in the Trypanothione-Based Redox Metabolism of Pathogenic Trypanosomes.

Abstract : Abstract Significance: Glutaredoxins are ubiquitous small thiol proteins of the thioredoxin-fold superfamily. Two major groups are distinguished based on their active sites: the dithiol (2-C-Grxs) and the monothiol (1-C-Grxs) glutaredoxins with a CXXC and a CXXS active site motif, respectively. Glutaredoxins are involved in cellular redox and/or iron sulfur metabolism. Usually their functions are closely linked to the glutathione system. Trypanosomatids, the causative agents of several tropical diseases, rely on trypanothione as principal low molecular mass thiol, and their glutaredoxins readily react with the unique bis(glutathionyl) spermidine conjugate. Recent Advances: Two 2-C-Grxs and three 1-C-Grxs have been identified in pathogenic trypanosomatids. The 2-C-Grxs catalyze the reduction of glutathione disulfide by trypanothione and display reductase activity towards protein disulfides, as well as protein-glutathione mixed disulfides. In vitro, all three 1-C-Grxs as well as the cytosolic 2-C-Grx of Trypanosoma brucei can complex an iron-sulfur cluster. Recently the structure of the 1-C-Grx1 has been solved by NMR spectroscopy. The structure is very similar to those of other 1-C-Grxs, with some differences in the loop containing the conserved cis-Pro and the surface charge distribution. Critical Issues: Although four of the five trypanosomal glutaredoxins proved to coordinate an iron-sulfur cluster in vitro, the physiological role of the mitochondrial and cytosolic proteins, respectively, has only started to be unraveled. Future Directions: The use of trypanothione by the glutaredoxins has established a novel role for this parasite-specific dithiol. Future work should reveal if these differences can be exploited for the development of novel antiparasitic drugs. Antioxid. Redox Signal. 00, 000-000.
Document type :
Journal articles
Complete list of metadatas

Cited literature [81 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00844780
Contributor : Mariella Botta <>
Submitted on : Monday, July 15, 2013 - 8:53:01 PM
Last modification on : Friday, October 16, 2020 - 12:00:04 PM
Long-term archiving on: : Wednesday, April 5, 2017 - 12:23:22 PM

File

ars_2E2012_2E4932.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Marcelo A Comini, R Luise Krauth-Siegel, Massimo Bellanda. Mono- and Dithiol Glutaredoxins in the Trypanothione-Based Redox Metabolism of Pathogenic Trypanosomes.. Antioxidants and Redox Signaling, Mary Ann Liebert, 2012, 19 (7), pp.708-722. ⟨10.1089/ars.2012.4932⟩. ⟨pasteur-00844780⟩

Share

Metrics

Record views

234

Files downloads

329