Mycobacterium tuberculosis Beijing Genotype Is Associated with HIV Infection in Mozambique. - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2013

Mycobacterium tuberculosis Beijing Genotype Is Associated with HIV Infection in Mozambique.

Résumé

The Beijing genotype is a lineage of Mycobacterium tuberculosis that is distributed worldwide and responsible for large epidemics, associated with multidrug-resistance. However, its distribution in Africa is less understood due to the lack of data. Our aim was to investigate the prevalence and possible transmission of Beijing strains in Mozambique by a multivariate analysis of genotypic, geographic and demographic data. A total of 543 M. tuberculosis isolates from Mozambique were spoligotyped. Of these, 33 were of the Beijing lineage. The genetic relationship between the Beijing isolates were studied by identification of genomic deletions within some Regions of Difference (RD), Restriction Fragment Length Polymorphism (RFLP) and Mycobacterial Interspersed Repetivie Unit - variable number tandem repeat (MIRU-VNTR). Beijing strains from South Africa, representing different sublineages were included as reference strains. The association between Beijing genotype, Human Immunodeficiency Virus (HIV) serology and baseline demographic data was investigated. HIV positive serostatus was significantly (p=0.023) more common in patients with Beijing strains than in patients with non-Beijing strains in a multivariable analysis adjusted for age, sex and province (14 (10.9%) of the 129 HIV positive patients had Beijing strains while 6/141 (4.3%) of HIV negative patients had Beijing strains). The majority of Beijing strains were found in the Southern region of Mozambique, particularly in Maputo City (17%). Only one Beijing strain was drug resistant (multi-drug resistant). By combined use of RD and spoligotyping, three genetic sublineages could be tentatively identified where a distinct group of four isolates had deletion of RD150, a signature of the "sublineage 7" recently emerging in South Africa. The same group was very similar to South African "sublineage 7" by RFLP and MIRU-VNTR, suggesting that this sublineage could have been recently introduced in Mozambique from South Africa, in association with HIV infection.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
journal.pone.0071999.pdf (1.64 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

pasteur-00870731 , version 1 (05-12-2013)

Identifiants

Citer

Sofia O Viegas, Adelina Machado, Ramona Groenheit, Solomon Ghebremichael, Alexandra Pennhag, et al.. Mycobacterium tuberculosis Beijing Genotype Is Associated with HIV Infection in Mozambique.. PLoS ONE, 2013, 8 (8), pp.e71999. ⟨10.1371/journal.pone.0071999⟩. ⟨pasteur-00870731⟩
71 Consultations
289 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More