Genetic variability of genotype 1 hepatitis C virus isolates from Tunisian haemophiliacs.

Ahlem Djebbi 1, * Olfa Bahri 1 Houda Langar 1 Amel Sadraoui 1 Selma Mejri 1 Henda Triki 1
* Corresponding author
1 Institut Pasteur de Tunis
Abstract : This paper reports hepatitis C virus (HCV) prevalence, genotypes and phylogenetic characteristics in 95 haemophilic Tunisian patients. The studied population included 3 groups of patients according to their date of birth: before 1985 when inactivation procedures for clotting factors was introduced, between 1985 and 1994 when systematic anti-HCV screening of Tunisian blood donors was introduced and after this date. HCV infection was assessed by serological and molecular commercial tests. Genotypes were determined using the INNO-LiPA HCV test and by partial sequencing in the NS5b genomic region. Phylogenetic analyses were performed by comparing NS5b sequences of Tunisian haemophiliacs to published sequences. HCV infection was detected in 50.5% of cases with a significant decrease according to age. Subtype la was the most prevalent followed by subtype 1b (52.6% vs 44.7%); it was more frequent among haemophiliacs born before 1985. NS5b sequences were different from those obtained from non-haemophilic Tunisian patients and showed nucleic affiliation with HCV isolates from the USA. These findings suggest an infection through clotting factors imported to Tunisia and frequently manufactured from US blood donors. In contrast, subtype 1b showed approximately the same distribution among patients born before and after 1985; NS5b sequences from haemophiliacs were randomly distributed among other Tunisian sequences, favouring a transmission through cryoprecipitates prepared from Tunisian blood donors.
Keywords : Phylogeny Haemophiliacs Blood products Transmission HCV genotypes Molecular epidemiology
Document type :
Journal articles
Complete list of metadatas
https://hal-riip.archives-ouvertes.fr/pasteur-00871748
Contributor : Institut Pasteur Tunis <>
Submitted on : Thursday, October 10, 2013 - 12:11:49 PM
Last modification on : Wednesday, October 16, 2019 - 1:59:39 PM

Identifiers

  • HAL Id : pasteur-00871748, version 1
  • PUBMED : 19123302

Collections

RIIP | RIIP_TUNIS

Citation

Ahlem Djebbi, Olfa Bahri, Houda Langar, Amel Sadraoui, Selma Mejri, et al.. Genetic variability of genotype 1 hepatitis C virus isolates from Tunisian haemophiliacs.. Microbiologica -Bologna-, Luigi Ponzio e Figlio Editori, 2008, 31 (4), pp.473-480. ⟨pasteur-00871748⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

99

Contact

CCSD