Activity of subinhibitory concentrations of dapsone alone and in combination with cell-wall inhibitors against Mycobacterium avium complex organisms.

N. Rastogi 1, * K. S. Goh 1 V. Labrousse 1
* Corresponding author
Abstract : MICs of dapsone (p-p'-diaminodiphenylsulfone) were determined radiometrically for ten strains each of the Mycobacterium avium complex (MAC) and Mycobacterium tuberculosis. MICs ranged from 50 to 250 micrograms/ml for Mycobacterium tuberculosis and from 2 to 100 micrograms/ml for MAC. However, at a concentration as low as 1.5 micrograms/ml dapsone significantly inhibited growth of MAC bacteria when used in combination with other drugs specifically acting at the mycobacterial cell-wall level. The latter drugs were used in subinhibitory concentrations, and included m-fluorophenylalanine (an inhibitor of mycoside-C biosynthesis), ethambutol (an inhibitor of arabinogalactan biosynthesis), and ethionamide (an inhibitor of mycolic acid biosynthesis). Using a radiometric method (Bactec 460-TB), the activity of dapsone was found to be enhanced for 2/10 strains in the presence of m-fluorophenylalanine, for 3/10 strains in the presence of ethambutol and for 5/10 strains in the presence of ethionamide. A satisfactory correlation between the radiometric data and bacterial viable counts was established.
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Journal articles
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Submitted on : Wednesday, January 8, 2014 - 3:43:20 AM
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  • HAL Id : pasteur-00925366, version 1
  • PUBMED : 8187794

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N. Rastogi, K. S. Goh, V. Labrousse. Activity of subinhibitory concentrations of dapsone alone and in combination with cell-wall inhibitors against Mycobacterium avium complex organisms.. European Journal of Clinical Microbiology and Infectious Diseases, Springer Verlag, 1993, 12 (12), pp.954-8. ⟨pasteur-00925366⟩

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