Activities of roxithromycin used alone and in combination with ethambutol, rifampin, amikacin, ofloxacin, and clofazimine against Mycobacterium avium complex.

Abstract : Preliminary studies showed that roxithromycin possessed significant in vitro activity against a variety of atypical mycobacteria such as the Mycobacterium avium complex, M. scrofulaceum, M. szulgai, M. malmoense, M. xenopi, M. marinum, and M. kansasii and rare pathogens such as M. chelonae and M. fortuitum. In this investigation, radiometric MICs of roxithromycin, ethambutol, rifampin, amikacin, ofloxacin, and clofazimine for 10 clinical isolates of the M. avium complex (5 each from human immunodeficiency virus [HIV]-positive and HIV-negative patients) were determined. Roxithromycin MICs against all the isolates were below the reported maximum concentration of drug in serum at the routine pH of 6.8, and the MICs were further lowered by 1 to 2 dilutions at a pH of 7.4. In vitro enhancement of roxithromycin activity against all strains was further investigated by the previously established Bactec 460-TB method by combining the drugs at sub-MIC levels. Antibacterial activity of roxithromycin was enhanced in all 10 strains by ethambutol, in 3 strains each by rifampin and clofazimine, in 2 strains by amikacin, and in 1 strain by ofloxacin. In vitro screening of three-drug combinations showed that combinations of roxithromycin, ethambutol, and a third potential anti-M. avium drug (rifampin, amikacin, ofloxacin, or clofazimine) resulted in further enhancement of activity in 13 out of 20 drug combinations screened.
Document type :
Journal articles
Complete list of metadatas

Cited literature [23 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00925370
Contributor : Nalin Rastogi <>
Submitted on : Wednesday, January 8, 2014 - 4:11:09 AM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM
Long-term archiving on : Tuesday, April 8, 2014 - 10:16:22 PM

File

PMID_8092850_AAC-1994-Rastogi-...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

N. Rastogi, K. S. Goh, A. Bryskier. Activities of roxithromycin used alone and in combination with ethambutol, rifampin, amikacin, ofloxacin, and clofazimine against Mycobacterium avium complex.. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 1994, 38 (6), pp.1433-8. ⟨10.1128/AAC.38.6.1433⟩. ⟨pasteur-00925370⟩

Share

Metrics

Record views

123

Files downloads

229