Immunomodulatory spectrum of lipids associated with Mycobacterium avium serovar 8. - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue Infection and Immunity Année : 1995

Immunomodulatory spectrum of lipids associated with Mycobacterium avium serovar 8.

Résumé

Lipid fractions obtained from Mycobacterium avium serovar 8 were assessed for the ability to affect various immune functions of human peripheral blood mononuclear cells (PBM). Lipids included a total lipid fraction and fractions eluted from silicic acid column separation of that total lipid fraction, using chloroform and chloroform-methanol combinations. Lipid fractions were assayed for total carbohydrate and total 6-deoxyhexose content and were assessed for the ability to influence human macrophage function and the capacity to induce secretion of prostaglandin E2 (PGE2) and tumor necrosis factor alpha in PBM. The total lipid and serovar-specific glycopeptidolipid (GPL) fractions both induced significant levels of tumor necrosis factor alpha, as well as PGE2, in PBM exposed to a sublethal concentration of 100 micrograms lipid per 2 x 10(6) cells. In addition, the same concentrations of the 5 to 7% and GPL fractions induced significant levels of leukotriene B4 in PBM. Comparison of carbohydrate and 6-deoxyhexose contents of each fraction suggested a relationship to carbohydrate content and ability of fractions to induce immune modulator secretion. Analysis of GPL fractions from M. avium serovars 4 and 20 revealed that those GPL lacked the ability to induce PGE2. These results are explained by considering the difference in the carbohydrate residues of the oligosaccharide moieties.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
PMID_7806348_Infect_Immun_1995_Barrow_126-33.pdf (213.3 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

pasteur-00925373 , version 1 (08-01-2014)

Identifiants

Citer

W. W. Barrow, T. L. Davis, E. L. Wright, V. Labrousse, M. Bachelet, et al.. Immunomodulatory spectrum of lipids associated with Mycobacterium avium serovar 8.. Infection and Immunity, 1995, 63 (1), pp.126-33. ⟨pasteur-00925373⟩
88 Consultations
163 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More