Monoclonal infection involving Mycobacterium avium presenting with three distinct colony morphotypes. - Archive ouverte HAL Access content directly
Journal Articles Journal of Clinical Microbiology Year : 1996

Monoclonal infection involving Mycobacterium avium presenting with three distinct colony morphotypes.

(1) , (2) , (3) , (1)
1
2
3

Abstract

Recent reports indicate that polyclonal infections may play an important role in multiple drug resistance in Mycobacterium avium infections. We report here on the isolation of a single M. avium strain that appeared to have smooth colony morphology upon initial isolation on a Lowenstein-Jensen slant. Primary subculture onto Middlebrook 7H10, however, revealed three distinct morphotypes representing smooth opaque (SmO), smooth transparent (SmT), and rough (Rg) colony morphologies. All three morphotypes were identified as M. avium by standard biochemical procedures, Genprobe analysis, and mycolic acid patterns. Subsequent restriction fragment length polymorphism analysis, using SalI- and PvuII-digested genomic DNA, revealed identical patterns for hybridization with the IS1245 probe. Thin-layer chromatographic analysis of lipids from the three morphotypes revealed that only the SmT morphotype possessed what appeared to be lipid components similar to, but unlike, previously described serovar-specific glycopeptidolipid antigens. Further analysis of internally radiolabeled deacylated lipids from the SmT morphotype, by high-performance liquid chromatography and thin-layer chromatography, disclosed that some of these components can be internally radiolabeled with [14C] phenylalanine and [14C]mannose. These results suggest that these components are structurally similar to previously described glycopeptidolipid antigens. This is apparently the first report of a monoclonal infection involving a single strain of M. avium presenting with all three colony morphotypes, SmO, SmT, and Rg.
Fichier principal
Vignette du fichier
PMID_8880503_JCM_1996_Wright_2475-8.pdf (151.12 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

pasteur-00926646 , version 1 (10-01-2014)

Identifiers

  • HAL Id : pasteur-00926646 , version 1
  • PUBMED : 8880503

Cite

E. L. Wright, S. Zywno-van Ginkel, N. Rastogi, W. W. Barrow. Monoclonal infection involving Mycobacterium avium presenting with three distinct colony morphotypes.. Journal of Clinical Microbiology, 1996, 34 (10), pp.2475-8. ⟨pasteur-00926646⟩
28 View
78 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More