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A and B) Results for isolates that were clustered by neither IS6110-RFLP nor spoligotyping. (A) Lanes: A, molecular weight (MW) marker; B, negative control; C to I, various unclustered isolates (B) Lanes: A, MW marker; B to H, various unclustered isolates); I, negative control; J, an unclustered isolate, GP29, that did not generate any bands upon DRE-PCR; K, isolate B4 from cluster B; L, unclustered isolate GP37; M, MW marker. (C) DRE-PCR analysis of isolates that were clustered by IS6110-RFLP analysis followed by spoligotyping, FIG. 1. Representative patterns obtained following DRE-PCR analysis of M. tuberculosis clinical isolates Lanes: A to E, five isolates from four patients of cluster B; F, an unclustered isolate ,
H, an isolate of cluster C. DNA MW markers IX (A and B) and VI (C), from Boehringer Mannheim ,