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IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.

Abstract : Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered.
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  • HAL Id : pasteur-00931158, version 1
  • PUBMED : 9738067
  • PUBMEDCENTRAL : PMC105111

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D. van Soolingen, J. Bauer, V. Ritacco, S. Cardoso Leão, I. Pavlik, et al.. IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.. Journal of Clinical Microbiology, American Society for Microbiology, 1998, 36 (10), pp.3051-3054. ⟨pasteur-00931158⟩

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