Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype.

Igor Mokrousov 1, * Anna Vyazovaya 2 Viacheslav Zhuravlev 2 Tatiana Otten 2 Julie Millet 3 Wei-Wei Jiao 4 A-Dong Shen 4 Nalin Rastogi 3 Boris Vishnevsky 2 Olga Narvskaya 1
* Corresponding author
1 Institut Pasteur de Saint-Pétersbourg
2 Laboratory of Etiological Diagnostics
3 Institut Pasteur de la Guadeloupe
4 Key Laboratory of Major Diseases in Children
Abstract : : Mycobacterium tuberculosis Beijing genotype strains are rapidly disseminating, frequently hypervirulent and multidrug resistant. Here, we describe a method of their rapid detection by real-time PCR targeting the specific IS6110 insertion in the dnaA-dnaN genome region. The method was evaluated with both geographically and genetically diverse collection representing Beijing genotype-endemic and epidemic areas in East Asia and former Soviet Union i.e., major foci of its global propagation, and with clinical specimens.
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Journal articles
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https://hal-riip.archives-ouvertes.fr/pasteur-00947146
Contributor : Nalin Rastogi <>
Submitted on : Friday, February 14, 2014 - 5:09:23 PM
Last modification on : Monday, October 8, 2018 - 5:44:06 PM

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Igor Mokrousov, Anna Vyazovaya, Viacheslav Zhuravlev, Tatiana Otten, Julie Millet, et al.. Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype.. Journal of Clinical Microbiology, American Society for Microbiology, 2014, epub ahead of print. ⟨10.1128/JCM.03193-13⟩. ⟨pasteur-00947146⟩

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