H4K16 acetylation affects recombination and ncRNA transcription at rDNA in Saccharomyces cerevisiae. - Archive ouverte HAL Access content directly
Journal Articles Molecular Biology of the Cell Year : 2012

H4K16 acetylation affects recombination and ncRNA transcription at rDNA in Saccharomyces cerevisiae.

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Abstract

Transcription-associated recombination is an important process involved in several aspects of cell physiology. In the ribosomal DNA (rDNA) of Saccharomyces cerevisiae, RNA polymerase II transcription-dependent recombination has been demonstrated among the repeated units. In this study, we investigate the mechanisms controlling this process at the chromatin level. On the basis of a small biased screening, we found that mutants of histone deacetylases and chromatin architectural proteins alter both the amount of Pol II-dependent noncoding transcripts and recombination products at rDNA in a coordinated manner. Of interest, chromatin immunoprecipitation analyses in these mutants revealed a corresponding variation of the histone H4 acetylation along the rDNA repeat, particularly at Lys-16. Here we provide evidence that a single, rapid, and reversible posttranslational modification-the acetylation of the H4K16 residue-is involved in the coordination of transcription and recombination at rDNA.
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pasteur-00952222 , version 1 (26-02-2014)

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Elisa Cesarini, Anna d'Alfonso, Giorgio Camilloni. H4K16 acetylation affects recombination and ncRNA transcription at rDNA in Saccharomyces cerevisiae.. Molecular Biology of the Cell, 2012, 23 (14), pp.2770-81. ⟨10.1091/mbc.E12-02-0095⟩. ⟨pasteur-00952222⟩

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