 |
 |
Journal articles
Poly(ADP-ribosyl)ation acts in the DNA demethylation of mouse primordial germ cells also with DNA damage-independent roles.
Abstract : Poly(ADP-ribosyl)ation regulates chromatin structure and transcription driving epigenetic events. In particular, Parp1 is able to directly influence DNA methylation patterns controlling transcription and activity of Dnmt1. Here, we show that ADP-ribose polymer levels and Parp1 expression are noticeably high in mouse primordial germ cells (PGCs) when the bulk of DNA demethylation occurs during germline epigenetic reprogramming in the embryo. Notably, Parp1 activity is stimulated in PGCs even before its participation in the DNA damage response associated with active DNA demethylation. We demonstrate that PARP inhibition impairs both genome-wide and locus-specific DNA methylation erasure in PGCs. Moreover, we evidence that impairment of PARP activity causes a significant reduction of expression of the gene coding for Tet1 hydroxylases involved in active DNA demethylation. Taken together these results demonstrate new and adjuvant roles of poly(ADP-ribosyl)ation during germline DNA demethylation and suggest its possible more general involvement in genome reprogramming.
Cited literature [80 references]
https://hal-riip.archives-ouvertes.fr/pasteur-00955893
Contributor : Istituto Pasteur Fondazione Cenci Bolognetti <>
Submitted on : Wednesday, March 5, 2014 - 3:12:48 PM
Last modification on : Thursday, July 11, 2019 - 2:10:07 PM
Long-term archiving on: : Thursday, June 5, 2014 - 11:40:37 AM
Contributor : Istituto Pasteur Fondazione Cenci Bolognetti <>
Submitted on : Wednesday, March 5, 2014 - 3:12:48 PM
Last modification on : Thursday, July 11, 2019 - 2:10:07 PM
Long-term archiving on: : Thursday, June 5, 2014 - 11:40:37 AM
File
Ciccaroneetal2012.pdf
Publisher files allowed on an open archive