Identification of side population cells in mouse primordial germ cells and prenatal testis. - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue International Journal of Developmental Biology Année : 2011

Identification of side population cells in mouse primordial germ cells and prenatal testis.

Résumé

In mammals, the stem cells of spermatogenesis are derived from an embryonic cell population called primordial germ cells (PGCs). Spermatogonial stem cells displaying the "side population" (SP) phenotype have been identified in the immature and adult mouse testis, but noting is known about the expression of the SP phenotype during prenatal development of germ cells. The SP phenotype, defined as the ability of cells to efflux fluorescent dyes such as Hoechst, is common to several stem/progenitor cell types. In the present study, we analyzed and characterized the Hoechst SP via cytofluorimetric analysis of disaggregated gonads at different time points during embryonic development in mice. To directly test the hypothesis that the SP phenotype is a feature of germ cell lineage, experiments were performed on transgenic animals expressing enhanced green fluorescent protein (EGFP) under the control of the Oct4 promoter, to identify early germ cells up to PGCs. We found that prenatal gonads contain a fraction of SP cells at each stage analyzed, and the percentage of cells in the SP fraction decreases as development proceeds. Surprisingly, more than 50% of the PGCs displayed the SP phenotype at 11.5 dpc (days post coitum). The percentage of germ cells with the SP phenotype decreased steadily with development, to less than 1% at 18.5 dpc. Cytofluorimetric analysis along with immunocytochemistry performed on sorted cells indicated that the SP fraction of prenatal gonads, as in the adult testis, was heterogeneous, being composed of both somatic and germ cells. Both cell types expressed the ABC transporters Abcg2, Abcb1a, Abcb1b and Abcc1. These findings provide evidence that the SP phenotype is a common feature of PGCs and identifies a subpopulation of fetal testis cells including prospermatogonia whose differentiation fate remains to be investigated.
Fichier sous embargo
Fichier sous embargo
Date de visibilité indéterminée
Loading...

Dates et versions

pasteur-00978711 , version 1 (14-04-2014)

Identifiants

Citer

Maria-Lucia Scaldaferri, Stefania Fera, Laura Grisanti, Massimo Sanchez, Mario Stefanini, et al.. Identification of side population cells in mouse primordial germ cells and prenatal testis.. International Journal of Developmental Biology, 2011, 55 (2), pp.209-14. ⟨10.1387/ijdb.092977ms⟩. ⟨pasteur-00978711⟩

Collections

RIIP RIIP_FCB
53 Consultations
2 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More