Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

The protein tyrosine phosphatase SHP-1 regulates phagolysosome biogenesis.

Abstract : The process of phagocytosis and phagosome maturation involves the recruitment of effector proteins that participate in phagosome formation and in the acidification and/or fusion with various endocytic vesicles. In the current study, we investigated the role of the Src homology region 2 domain-containing phosphatase 1 (SHP-1) in phagolysosome biogenesis. To this end, we used immortalized bone marrow macrophages derived from SHP-1-deficient motheaten mice and their wild-type littermates. We found that SHP-1 is recruited early and remains present on phagosomes for up to 4 h postphagocytosis. Using confocal immunofluorescence microscopy and Western blot analyses on purified phagosome extracts, we observed an impaired recruitment of lysosomal-associated membrane protein 1 in SHP-1-deficient macrophages. Moreover, Western blot analyses revealed that whereas the 51-kDa procathepsin D is recruited to phagosomes, it is not processed into the 46-kDa cathepsin D in the absence of SHP-1, suggesting a defect in acidification. Using the lysosomotropic agent LysoTracker as an indicator of phagosomal pH, we obtained evidence that in the absence of SHP-1, phagosome acidification was impaired. Taken together, these results are consistent with a role for SHP-1 in the regulation of signaling or membrane fusion events involved in phagolysosome biogenesis.
Document type :
Journal articles
Complete list of metadata

Cited literature [69 references]  Display  Hide  Download
Contributor : Nancy Laflamme Connect in order to contact the contributor
Submitted on : Wednesday, June 4, 2014 - 7:35:52 PM
Last modification on : Thursday, June 18, 2020 - 12:32:05 PM
Long-term archiving on: : Tuesday, April 11, 2017 - 3:47:13 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Carolina P Gómez, Marina Tiemi Shio, Pascale Duplay, Martin Olivier, Albert Descoteaux. The protein tyrosine phosphatase SHP-1 regulates phagolysosome biogenesis.. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2012, 189 (5), pp.2203-10. ⟨10.4049/jimmunol.1103021⟩. ⟨pasteur-01001767⟩



Record views