1. Can J Microbiol. 2012 Oct;58(10):1221-9. doi: 10.1139/w2012-101. Epub 2012 Oct

Deletion of TerD-domain-encoding genes: effect on Streptomyces coelicolor

Sanssouci É(1), Lerat S, Daigle F, Grondin G, Shareck F, Beaulieu C.

Author information: 
(1)Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de
biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.

TerD-domain-encoding genes (tdd genes) are highly represented in the Streptomyces
coelicolor genome. One of these, the tdd8 gene, was recently shown to have a
crucial influence on growth, differentiation, and spore development of this
filamentous bacterium. The investigation of the potential role of tdd genes has
been extended here to tdd7 (SCO2367) and tdd13 (SCO4277). Both genes are highly
expressed in bacteria grown in liquid-rich medium (tryptic soy broth). However,
the deletion of these genes in S. coelicolor showed contrasting effects regarding
developmental patterns, sporulation, and antibiotic production. Deletion of the
tdd7 gene induced a reduction of growth in liquid medium, wrinkling of the
mycelium on solid medium, and poor spore and actinorhodin production. On the
other hand, deletion of the tdd13 gene did not significantly affect growth in
liquid medium but induced a small colony phenotype on solid medium with abundant 
sporulation and overproduction of undecylprodigiosin. Although their exact
functions remain undefined, the present data suggest a major involvement of TerD 
proteins in the proper development of S. coelicolor.

PMID: 23072443  [PubMed - indexed for MEDLINE]